抗肿瘤坏死因子单克隆抗体对持续缺血和缺血再灌注损伤心肌细胞凋亡的影响

The effect of anti-TNF-mAb on the apoptosis of myocardial cells during the continuous ischemia and ischemia reperfusion injury

目的:探讨抗肿瘤坏死因子单克隆抗体对持续缺血和缺血再灌注损伤心肌细胞凋亡的影响。方法:采用大白鼠随机分为7组。用冠脉结扎法,建立急性心肌梗死动物模型。以活结结扎左冠状动脉的前降支,分别造成阻断冠脉血流和再灌注。以TUNEL标记凋亡细胞。结果:凋亡细胞原位标记与半定量分析表明:抗肿瘤坏死因子单克隆抗体处理组与持续缺血组、缺血再灌注组比较心肌细胞凋亡程度明显降低。结论:缺血再灌注损伤和持续缺血可导致心肌细胞凋亡,抗肿瘤坏死因子单克隆抗体预处理能明显减轻心肌细胞凋亡程度。

Objective： To study the effect of anti-TNF-mAb on the apoptosis of myocardial cells during the continuous ischemia and the ischemia reperfusion injury. Methods：Wistar rats were randomly divided into 7 groups. Acute myocardial infarction animal models were formed by ligating the coronary artery. L The anterior descending branches of the left coronary artery was ligated with slipknot to block the blood flow and reperfuse the myocardium. The apoptotic cell was labeled by TUNEL. Results ：In situ apoptotic cell labeling and semiquantitative analysis reveal ： The degree of the apoptosis of myocardial, eel.is decreased significantly in the anti-TNF-mAb-administrated group compared with continuous ischemia group and ischemia reperfusion injury group. Conclusion： Continuous ischemia and ischemia reperfusion injury can result in the apoptosis of myocardial cells; the preconditioning with anti-TNF-mAb can lessen the degree of the apoptosis of myocardial cells signigicantly.