艾灸预处理对佐剂性关节炎大鼠下丘脑HSP70的影响及保护机制研究

Influence of moxibustion preconditioning on hypothalamic HSP70 in rats with adjuvant arthritis and its protective mechanism

目的以往研究证实艾灸预处理对随后佐剂性关节炎(Adjuvantarthritis,AA)大鼠具有一定的阻抑保护作用。本研究进一步从下丘脑HSP70角度,探询艾灸预处理保护作用的可能机制。方法应用免疫组织化学的方法,用AA大鼠模型,观察艾灸预处理在大鼠造模前、造模后早期及继发期下丘脑HSP70的变化,并应用图像分析技术进行综合分析。结果正常组大鼠下丘脑有少量HSP70的阳性表达。与正常组相比,AA各组大鼠早期和继发期下丘脑HSP70呈强阳性表达。但随疾病进程呈缓降的趋势,与同期AA组相比,经过艾灸预处理各组可使AA大鼠HSP70异常高表达的状况得到缓解,无论早期还是继发期均较明显。艾灸预处理还可使正常大鼠下丘脑HSP70的表达增强。结论艾灸预处理对随后AA大鼠的保护作用可能与其适度提高正常大鼠的下丘脑HSP70的阳性表达,以及明显减弱随后AA大鼠下丘脑HSP70的异常高表达有关。下丘脑中HSP70可能是艾灸预处理产生防病减病、保健强壮的重要分子之一。

Objective The primary study has confirmed that moxibustion preconditioning （MP） had a protective action on rats with adjuvant arthritis （AA）. This paper investigated the protective mechanism of MP through an analysis of hypothalamic HSP70. Methods To observe the changes of hypothalamic HSP70 in AA rats before model established, at early stage and secondary stage after model established respectively by using immunohistochemistry, and to analyze these changes with image analysis technique. Results The expression of hypothalamic HSP70 was limited positive in the normal group, and that in AA group of early stage and secondary stage was strong positive compared with the normal group, but had a decreasing trend along with the disease course. In the groups with MP the abnormal high expressions of hypothalamic HSP70 were relived not only at early stage but also at secondary stage compared with AA group. MP also improved the expression of hypothalamic HSP70 in normal rats. Conclusion The protective action of MP on AA rats may be related to that it can improve the positive expression of hypothalamic HSP70 in normal rats, and decreases obviously the abnormal high expression of hypothalamic HSP70 in AA rats. Hypothalamic HSP70 may be one of important factors for MP actions of preventing and relieving the disease and strengthening the constitution.