摘要
在分批发酵中,研究了Klebsiellapneumoniae的生长、底物甘油消耗及1,3?丙二醇的产生特性.基于Logistic方程和Luedeking?Piret方程,得到了描述1,3?丙二醇分批发酵过程的动力学模型及模型参数,该组模型能很好地拟合发酵过程,并在初始甘油浓度变化较大的范围内表现出很好的适用性.同时,所建立的模型也基本反映了Klebsiellapneumoniae分批发酵过程的动力学特征.基于分批发酵动力学模型,提出了以甘油为单一碳源时的底物流加策略,通过与其他流加策略条件下的发酵对比实验表明,通过基于动力学模型的流加策略可获得更高的1,3?丙二醇浓度及生产强度.
The fermentation process of 1,3-propanediol by Klebsiella pneumoniae with glycerol as substrate was studied in batch system. A kinetic model was proposed based on the Logistic and Luedeking-Piret equations of cell growth, product formation and substrate consumption. With the evaluated model parameters, the model could provide reasonable description for the fermentation process under various conditions. Based on the model, a feeding strategy for glycerol was determined to maximize the final 1,3-propanediol concentration. The experimental results showed that the feeding mode with nonlinear optimization could improve the 1,3-propanediol productivity and concentration compared with other feeding strategies, such as pulse feeding and constant glycerol concentration feeding.
出处
《过程工程学报》
EI
CAS
CSCD
北大核心
2005年第4期425-429,共5页
The Chinese Journal of Process Engineering
基金
'十五'国家科技攻关计划资助项目(编号:2001BA708B01-04)
