优势菌种硝化新工艺处理垃圾渗滤液的研究

Experimental research of landfill leachate treated by new type technics of nitrifying superiorbacterium

通过摇瓶富集与开放体系扩大培养获得高含量的硝化细菌优势菌液,用于硝化反应器的强化挂膜启动、驯化。采用优势菌种生物硝化新工艺研究了含高质量浓度氨氮垃圾渗滤液的硝化特性,并对工艺条件进行了优化。结果表明,优势菌液中亚硝化细菌与硝化细菌的含量分别达到9.0×107和3.5×107MPN/mL。实际废水动态运行的结果显示,当进水垃圾渗滤液平均氨氮质量浓度为284.4mg/L时,出水平均氨氮质量浓度为14.3mg/L,硝化速率高达NH4+-N28.1g/(m3.h),与传统硝化工艺相比高出近一倍。本工艺处理垃圾渗滤液的优化操作工艺条件为:pH、氨氮质量浓度及DO分别控制在7.5～8.5、300mg/L、1.1～2.6mg/L。

Nitrifying superiorbacterium enriched by open-culture flask-shaker was used to bioaugment the nitrifying biofilm growth in reactor during the start-up and acclimation periods, The nitrification characteristic of landfill leachate with high concentration ammonia nitrogen was studied by a new type technics of nitrifying superiorbacterium, further more, the technics parameters was optimized in this study. The result showed that the concentration of nitrite bacteria and nitrate bacteria in broth could achieve 9.0×10^7 and 3.5×10^7 MPN/mL respectively alter enrichment. With influent ammonia nitrogen concentration of 284.4 mg/L, the effluent ammonia of the nitrifying reactor is 14.3 mg/L.Comparing with no bioaugrnentation, the nitrification ratio of the new type technics is twice as many and could achieve NH4^＋-N 28.1 g/（m^3·h）.In addition, the optimization operational factor is controlled as follows： pH 7.5-8.5, NH4^＋-N 300 mg/L and DO 1.1-2.6 mg/L.