吻合血管同种异体骨移植术后微嵌合现象的研究

Microchimerism in recipients after vascularized allogratf bone transplantation

目的研究吻合血管同种异体骨移植术后受体微嵌合现象(microchimerism)的存在及其与免疫耐受的相关性。方法吻合血管同种异体大段股骨干移植术后不同时期进行X线和组织学切片检查,并对不同组织器官进行性别决定因子Y(Sry)半定量聚合酶链反应(PCR)分析。结果实验组在术后不同时期的X线及组织学表现为典型的骨折愈合模式;对照组X线及组织学表现为密度增高的移植骨被大量骨痂包绕,以及畸形愈合等排斥反应征象。Sry半定量PCR分析显示,实验组术后器官组织的微嵌合发生率及嵌合率明显高于对照组,且随术后期的延长而增高。结论吻合血管同种异体骨移植术后,受体的一些器官组织存在着微嵌合现象,且微嵌合发生率与受体对移植骨组织相容性呈正相关。

Objective To investigate the status of microchimerism in recipients and the relation between microchimerism and immunologic tolerance after vascularized allograft bone transplantation.Methods X-ray and histological examinations were performed on recipients after massive vascularized shaft of femur from female Japanese white rabbit donors was transplanted to male recipients. Microchimerism in different organs and tissues were checked postoperatively using a semi-quantitative polymerase chain reaction （PCR） with a Y-chromosome specific primer at different time. Results X-ray and histological examinations showed typical bone union in the experiment group but irregular new bone calluses surrounded the transplanted bones, with high density sequestrum in the control group. Semiquantitative PCR with a Y-chyomosome specific primer indicated that the incidence of microcbimerism in organs andtissues in the experiment group was higher than that in the control group postoperatively and increased with time. Conclusions After vascularized allograft bone transplantation, organs and tissues show microchimerism that has a positive correlation with the histocompatibility of the transplanted bones.