多点培养法分离培养150例甲真菌病病原菌

Isolation of Pathogenic Fungi from Patients with Onychomycosis by Multipoint Inoculation Method

目的比较多点培养法和常规培养法在甲真菌病病原菌分离中的差别;获取甲真菌病病原菌流行病学资料。方法150例病甲标本同时采用多点培养法和常规培养法分离菌种并作菌种鉴定。结果多点培养法与常规培养法的培养阳性率和菌种构成差异有统计学意义(P<0.05)。女性、手指甲、近端甲下型和低临床指数评分者病原菌以酵母菌为主;男性、足趾甲、远端侧位甲下型和高临床指数评分者病原菌中皮肤癣菌占优势。结论多点培养法阳性率高,能发现更多酵母菌。

Objectives To compare the difference between multipoint inoculation and routine method for isolation of pathogenic fungi from nail samples of onychomycosis, and to analyze the epidemiology of pathogenic fungi in those patients. Methods The nail clipping samples from each patient were inoculated onto the plates with Sabouraud＇s agar, Sabouraud＇s agar without cyeloheximide and medium containing rapeseed oil, respectively, by an approach of at least seven inoculating points in each plate （multipoint inoculation）, and onto medium slope in tuhes with the same media as above mentioned （routine method）. In the multipoint inoculation method, plates with more than 3 colonies were taken for further identification of pathogenie fungi based on morphological and biochemical properties. Results Based on the data from 150 samples of onychomycosis, significant differences were found between multipoint inoculation method and routine method （P 〈0.05） with respects of isolation rates （65.3% vs, 45.3%） and species constituent of dermatophytes （50.8% vs.73.2% ）, yeasts （46.7% vs. 26.8%） and moulds （2.5% vs. 0% ）. Yeasts were more likely to be isolated from the female, finger-nail, proximal subungual type and patients with lower scoring clinical index, while dermatophytes were more likely isolated from the male, toe-nail, distal lateral subungual type and patients with higher scoring clinical index. Conclusion Multipoint inoculation method seems to yield more yeasts than routine method, which is helpful for assessment of the constituent of pathogenic, fungi and their epidemiological properties in onychomycosis.