波氏假阿利什菌和尖端赛多孢子菌随机扩增DNA多态性分析

Characterization of Pseudallescheria boydii and Scedosporium apiospermum by Random Amplification of Polymorphic DNA Assay

目的了解波氏假阿利什菌和尖端赛多孢子菌的基因学特征,研究DNA分型与菌种来源的关系。方法采用随机扩增多态性DNA分析(RAPD)方法。结果3种引物可将来自5个国家的13株波氏假阿利什菌和18株尖端赛多孢子菌分为31个基因型。多引物聚类分析所得树状图显示,除来自哥伦比亚土壤的3株波氏假阿利什菌外,其他受试菌株无地域性群集分布特点。但受试菌株中的多数波氏假阿利什菌和尖端赛多孢子菌株分别聚集成一群。结论波氏假阿利什菌和尖端赛多孢子菌存在较大株间差异,致病菌没有明显的地域性分布趋势,RAPD分型聚类分析结果与形态学分类之间具有一定一致性。

Objective To investigate the DNA polymorphism of Pseudallescheria boydii and Scedosporium apiospermum and to analyze the relationship between random amplification of polymorphic DNA（RAPD） patterns and geographic origins. Methods The genomic DNAs of 13 Pseudallescheria boydii strains and 18 Scedosporium apiospermum strains isolated from 5 countries were amplified with RAPD technique. Results All strains tested were classified into 31 patterns by combination of the results obtained with 3 primers. The cluster tendency was identified based on species difference, namely, P. boydii or S. apiospermum strains, however, no such cluster tendency was revealed based on geographic origins of the most of strains, by dendrogram analysis. Conclusions The infraspecific variability of P. boydii and S. apiospermum is considerable. The cluster tendency of RAPD profiles is of consistency with morphological properties of P. boydii and S.apiospermum to some degree, however, is of no correlation with geographic origins of the pathogenic strains.