两种培养基体外诱导白念珠菌菌丝相形成的比较

Induction of Hyphal Form of Candida albicans in vitro by Two Culture Media

目的寻找白念珠菌菌丝相理想的体外培养条件。方法分别采用RPMI1640和DMEM培养基,在37℃条件下培养对氟康唑敏感性不同的16株白念珠菌,计算并比较白念珠菌菌丝相在两种培养基中的形成率。结果在DMEM培养基中白念珠菌菌丝相的形成率低于在RPMI1640培养基中的形成率。RPMI1640培养基(pH7.5),37℃传代培养7d(转种12次)后,16株白念珠菌菌丝相细胞形成率均达99%以上。在DMEM培养基中,于同一观察时间,氟康唑剂量依赖性敏感和耐药株的菌丝相形成率低于氟康唑敏感株。结论RPMI1640培养基(pH7.5),37℃传代培养7d(转种12次)是获得白念珠菌菌丝相的理想条件。

Objective To cmnpare 2 approaches with different culture media which induce hyphal form of Candida albicans. Methods Induction of hyphal form was conducted for 16 C. albicans strains with either RPMI 1640 medium or DMEM medium, at 37℃ for 24 h, respectively. The hyphal and yeast forms were counted separately and the ratio of hyphal form to total cells was calculated. Results The ratio of hyphal form to total cells was higher in RPMI 1640 medium than that in DMEM medium at the same incubation time for the majority of strains. The ratio was above 99% for all strains after 7-day incubation with 12 times of passages in RPMI 1640 medium at 37℃. Moreover the ratio of hyphal form was significantly higher for fluconazole-susceptible strains than that for fluconazole dose-dependent susceptible and resistant strains in incubation with DMEM medium at 37℃. Conclusion Incubation with RPMI 1640 medium at 37℃ for 7 days seems a favorahle condition to induce hyphal form of C. albicans.