外阴阴道念珠菌病小鼠模型阴道组织趋化因子MCP-1和MIP-2 mRNA水平测定

Expression of MCP-1 and MIP-2 mRNA in Vagina of Murine Model of Vulvovaginal Candidiasis

目的探讨趋化因子在白念珠菌性阴道炎模型小鼠阴道组织中的表达情况。方法采用雌激素化小鼠念珠菌性阴道炎模型,另以雌激素化但未接种白念珠菌组、接种等量白念珠菌但未雌激素化组为对照组,动态观察小鼠阴道灌洗液的真菌生长情况,并在接种后不同时间随机摘取小鼠的阴道组织,半定量RT-PCR方法检测组织内单核细胞趋化蛋白-1(MCP-1)、巨噬细胞炎性蛋白-2(MIP-2)mRNA的表达情况。结果与未雌激素化的小鼠比较,雌激素化并接种白念珠菌小鼠阴道灌洗液自第2天开始即可发现念珠菌生长并持续到接种后21d,自第4天起阴道组织内有高水平的MCP-1mRNA表达,并持续高于另外两组直到观察期末。3组小鼠的MIP-2mRNA表达水平在接种后第2天明显高于完全阴性对照组,但以后各时间点3组间两两比较差异无统计学意义。结论MCP-1mRNA高水平表达可能与白念珠菌持续的阴道感染过程有关,而MIP-2在感染过程中的作用不明显。

Objective To investigate the kinetic expression level of chemokines （MCP-1 and MIP-2）in vagina of a murine model of vulvovaginal candidiasis （VVC）, Methods The estrogen-treated murine model of VVC was set up. Vaginal specimens were obtained in different duration after inoculation of C. albicans intravaginally. Semi-quantitative RT-PCR was applied to determine MCP-1 and MIP-2 mRNA levels in these tissues. Results Compared with the control mice treated with olive oil, persistent growth of C.albicans was found from the 2nd day to 21st day after inoculation in estrogen-treated mice. Significantly higher levels of MCP-1 mRNA were observed in vaginal tissues in infected estrogen-treated mice than those in other 2 groups, infected but non estrogen-treated mice and estrogen-treated but uninfected mice. The high level of MCP-1 mRNA maintained from the 4th day to 21st day in infected estrogen-treated mice. It was also found that levels of MIP-2 mRNA were significantly higher in the vagina in the 2nd day in 3 groups of mice than those in naive mice, however, no significant difference was shown among 3 groups throughout the study period. Conclusion High level of MCP-1, rather than MIP-2, may be associated with susceptibility to VVC.