三七总皂苷对博莱霉素致大鼠肺纤维化的干预作用

Effect of Panax Notoginsenosides on pulmonary fibrosis induced by bleomycin in rats

目的:观察三七总皂苷对大鼠肺纤维化的干预作用,探讨其作用机制。方法:120只Wistar大鼠随机分为模型组、对照组、三七总皂苷组、地塞米松组。采用博莱霉素制备肺纤维化大鼠模型,24h后给予干预药物,分别于7、14、21、28天处死动物后观察其病理变化、肺系数、肺组织羟脯氨酸含量及转化生长因子β1的表达。结果:与模型组比较,三七总皂苷组与地塞米松组的肺泡炎和肺纤维化明显减轻,羟脯氨酸含量、肺组织TGFβ1表达降低(P<0.01或P<0.05)。结论:三七总皂苷能明显抑制博莱霉素诱导的大鼠肺纤维化的过程。

To observe the effect of Panax Notoginsenosides（PNS）on pulmonary fibrosis induced by bleomycin in rats and explore its mechanism. Methods： 120 Wistar rats were randomly divided into control group（CG）, model group （MG）, PNS group（PG） and dexamethasone group（DG）. After the model of pulmonary fibrosis was established by a single intratracheal instillation of bleomycin for 24 h, the above - mentioned drugs were given, respectively. On day 7, 14,21and 28 after treatment, 6 rats of each group were sacrificed, respectively, and their lungs were harvested for measurement of the lung index, pathology change, Masson dying, concentration of hydroxyproline（HyP）, and expression of TGF - β1 in lung tissues Results： PNS and dexarnethasone could obviously alleviated alveolitis and fibrosis induced by bleomycin. The concentration of HyP and expression of TGF - β1 in the lung tissues decreased in PG and DG compared with those in MG （P＜0.01 and 0.05, respectively）. Conclusion： PNS may obviously delay the development of pulmonary fibrosis induced by bleomycin in rats.