刺激剂对植物细胞悬浮培养的影响

Influence of elicitor on cell suspension cultures of plants

对刺激剂对植物细胞悬浮培养的影响及如何提高刺激剂的诱导率进行了综述。刺激剂对细胞悬浮培养的影响主要表现为酶活化、蛋白质含量改变、氧迸发、细胞程序性死亡、pH值改变、次生代谢产物积累等防御反应。提高刺激剂对悬浮培养细胞的诱导效率必须优化刺激剂的种类,刺激剂的浓度和加入时间,创建更好的诱导模式。

Influence of elicitor and how to enhance inductivity on cell suspension cultures are summarized in this paper. Defense responses are manifested mainly on cell suspension cultures by elicitor influence, such as enzyme activation, changes of protein content and pH, oxidative burst, programmed cell death,accumulation of secondary metabolites,et al. Elicitor species, concentration and induction phase ,and better induction model are very important to the improvement of inductivity on cell suspension cultures.