开心胶囊含药血清对血管紧张素Ⅱ诱导的心肌细胞肥大的影响

Effect of serum containing Kaixin capsule on myocyte hypertrophy induced by angiotensin Ⅱ

目的:应用BCA法检测含药血清中以血管紧张素Ⅱ诱导的心肌细胞肥大模型蛋白质的含量,观察开心胶囊对抗心室重构过程中心肌细胞肥大的效应。方法:实验于2001-03/2002-03广州中医药大学实验动物中心完成。①取雄性SD大鼠20只,随机分为5组,即正常组,普通模型组(不用药),西药组(以0.75g/L卡托普利,10mL/kg灌胃),开心胶囊等效剂量组(1400g/L开心胶囊,10mL/kg灌胃)及2倍剂量组(2800g/L开心胶囊,10mL/kg灌胃),每组4只,用于制备大鼠含药血清。②另选用1～3d内出生的SD乳鼠15只,用于分离心肌细胞,并对应上述5组血清分成5份,体外培养,向除正常组外的4组乳鼠心肌细胞中加入血管紧张素Ⅱ,造成心肌细胞肥大模型,心肌肥大时RNA转录和蛋白质合成增加,呈剂量和时间依赖性。③用BCA法检测普通模型组、西药组、中药开心胶囊等效剂量组及2倍剂量组血清中心肌细胞肥大模型蛋白质的含量,比较与正常组血清中正常心肌细胞蛋白质含量的差异,并做组间比较。结果:①普通模型组血清内心肌细胞蛋白质含量明显高于正常血清组犤(1725.34±105.05),(198.26±87.03)mg/L,t=27.53,P<0.01犦。②西药组、中药等效剂量组和中药2倍剂量组血清中蛋白质含量均明显低于普通模型组犤(1041.67±270.09),(1360.34±180.31),(1284.51±91.44),(1725.34±105.05)mg/L,t=5.81,4.92,7.54,P均<0.01犦。③等效剂量组与西药组比较差异具有显著性(t=2.41,P<0.05),2倍剂量组与西药组相比差异不显著(t=2.08,P>0.05),且2倍剂量组虽然较等效剂量组蛋白质含量降低,但无统计学意义(t=0.89,P>0.05)。结论:应用血管紧张素Ⅱ诱导新生大鼠心肌细胞肥大,其蛋白质合成增加;而用含药血清干预后,均有效地减少体外培养的心肌细胞蛋白质的合成,以等效剂量组效果明显。

AIM： To detect the content of protein in rat model of myocyte hypertrophy induced by angiotensin Ⅱ with BCA method and explore the effect of serum containing Kaixin capsule on the prevention of myocyte hypertrophy. METHODS： The experiment was done in the Animal Laboratory of Guangzhou University of Traditional Chinese Medicine, between March 2001 to March 2002, ①Twenty male SD rats were selected and divided randomly into five groups, normal group, model group （without administration）, western medicine group （0,75 g/L captopil, 10 mL/kg intragastrical administration）, equivalent dose group （1 400 g/L Kaixin capsule, 10 mL/kg intragastrical administration）, and double dose group （2 800 g/L Kaixin capsule, 10 mL/kg intragastrical administration）. There were 4 rats in each group. ② Another 15 SD newborn rats at 1-3 days were selected to isolate myocardial cells which were divided into five parts and cultured in vitro. Angiotensin 11 were administrated into myocardial cells of newborn rats except those in normal group to establish rat models of myocyte hypertrophy. An increase in RNA transcription and protein synthesis was found during myocyte hypertrophy in a dose-time dependent manner. ③ Contents of protein in the model group, western medicine group, equivalent dose group and double dose group were measured with BCA method, and the results were compared with that of the normal group. Meanwhile, comparison among five groups was performed. RESULTS： ① The content of protein in the model group was significantly higher than that in the normal group[（ 1 725.34±105.05）,（198.26±87.03 ） mg/L,t=27.53 ,P 〈 0,01]. ② The content of protein in the model group was significantly higher than that in the western medicine group, equivalent dose group and double dose group, respectively[（1 725.34± 105.05 ）, （ 1 041.67±270,09 ）, （ 1 360.34± 180.31 ）, （ 1 284.51±91.44 ） mg/L, t=5.81,4.92,7.54, P 〈 0.01], ③ Significant difference was found between the equivalent dose group and western medicine group （t=2.41, P 〈 0.05）, but not found between the double dose group and western medicine group （t=2.08, P 〉 0.05）, Though the content of protein in the equivalent dose group was decreased as compared with that in the double dose group, there was no significance between the two groups（t=0.89,P 〉 0,05）. CONCLUSION： Synthesis of protein is increased in the newborn rats with myocyte hypertrophy due to angiotensin Ⅱ; however, serum eontaining Kaixin capsule can decrease the synthesis of protein in myocardial cells in vitro, especially the equivalent dose group.