桑寄生对肾性高血压大鼠血浆β-内啡肽浓度影响的量效作用

Effect of Chinese taxillus twig on the concentration of plasma beta-endorphin in rats with renal hypertension in a dose-effect manner

目的:探讨降压作用明显的桑寄生对肾性高血压大鼠血浆β-内啡肽浓度的影响。方法:实验于2003-03/12在重庆医科大学动物实验中心完成。取Wistar大鼠60只,随机分5组,桑寄生细粉蒸馏水配制成浓度分别为200g/L和100g/L桑寄生组(100mL药液中分别含桑寄生药材细粉10g和20g)、卡托普利组(阳性对照组,蒸馏水配成10mL/L浓度)、蒸馏水组(模型组)和假手术组,每组12只。用“二肾一夹法”复制肾性高血压模型,术后分别以200g/L桑寄生、100g/L桑寄生、10mL/L卡托普利、蒸馏水20mL/kg灌胃,假手术组不进行肾动脉狭窄造模仅用蒸馏水灌胃。上述处理连续26d,于第27天测定血浆β-内啡肽浓度应用放射免疫法。结果:60只大鼠均进入结果分析。β-内啡肽浓度:①模型组明显高于假手术组犤(2.31±0.24,1.97±0.30)μg/L,(P<0.01)犦。②200g/L桑寄生组明显低于模型组犤(1.87±0.26,2.31±0.24)μg/L,(P<0.01)犦。③100g/L桑寄生组和卡托普利组低于模型组犤(2.09±0.28,2.31±0.24)μg/L,(2.08±0.22,2.31±0.24)μg/L,(P<0.05)犦。结论:桑寄生能降低模型大鼠血浆β-内啡肽浓度,该作用在200g/L时效果最好。

AIM： To investigate the influence of Chinese taxillus twig, which has an obvious effect on depression, on the plasma concentration of beta-endorphin in rats with renal hypertension. METHODS： The experiment was completed in the Animal Experiment Center of Chongqing University of Medical Sciences between March and December 2003. Sixty Wistar rats were randomly divided into 5 groups with 12 rats in each group： 200 and 100 g/L Chinese taxillus twig groups （100 drug fluid containing 20 g and 10 g Chinese taxillus twig powder respectively）, distilled water group （model group） and sham-operated group. Rat models of renal hypertension were replicated with the ＂two kidney one clip＂ method, and then they were treated with gastric perfusion of 200 and 100 g/L Chinese taxillus twig, 10 mL/L captopril, and 20 mL/kg distilled water respectively; Rats in the sham-operated group did not receive stenosis of renal artery, and were only treated with gastric perfusion of distilled water;, All the rats were treated for 26 continuous days; The plasma concentration of beta-endorphin was detected with radioimmunoassay on the 27^th day. RESULTS： All the 60 rats were involved in the analysis of results. Plasma concentration of beta-endophin： ① It was obviously higher in the model group than in the sham-operated group [（2.31±0.24）, （1.97±0.30） μg/L, P〈 0.01]; ② It was obviously lower in the 200 Chinese taxillus twig group than in the model group [（1.87 ±0.26）, （2.31±0.24） μg/L, P 〈 0.01]; ③ Those were lower than in the 100 Chinese taxillus twig group and captopril group than in the model group [（2.09±0.28）, （2.31±0.24） μg/L; （2.08±0.22）, （2.31±0.24） μg/L, P 〈 0.05]. CONCLUSION： Chinese taxillus twig can decrease the plasma concentration of beta-endorphin in model rats, and the effect is the best when the dosage was 200 g/L.