α-黏着斑蛋白在大鼠心脏表达分布随增龄变化的特征(英文)

Age-dependant expression of alpha-macula adherens protein in rat heart

背景:黏着斑蛋白与心脏的先天畸形发育和心肌细胞的分化有着密切的关系。目的:观察α-黏着斑蛋白在大鼠心脏的表达分布特征以及在心肌细胞发育过程中随增龄发生的变化规律。设计:随机对照,观察对比实验。单位:新乡医学院细胞生物学教研室;濮阳职业技术学院生物工程与农业经济系。材料:实验于2003-01/2003-06在新乡医学院形态学研究室完成。选取清洁级Wistar大鼠28只,按年龄分为幼年组、青年组、中年组、老年组,每组7只。方法:所有大鼠在麻醉状态下取大鼠心脏组织,冠状连续系列切片,片厚5μm。幼年、青年、中年和老年大鼠的心肌细胞α-黏着斑蛋白的表达应用免疫组化方法进行观测。在细胞表面、胞浆中及闰盘处出现棕黄色颗粒为阳性细胞。所有标本均做常规染色(苏木精-伊红染色)为结构对照。主要观察指标:各组大鼠心肌细胞α-黏着斑蛋白的表达。结果:28只大鼠均进入结果分析。①α-黏着斑蛋白在大鼠的心房、心室、乳头肌、室间隔等处的心肌细胞均有表达。②幼年时期α-黏着斑蛋白的免疫标记颗粒大部分集中在心肌细胞末端的闰盘处,少量分散地分布于细胞表面和胞浆中;青年、中年和老年大鼠α-黏着斑蛋白的免疫标记典型的分布在心肌细胞末端的闰盘处。结论:在大鼠心肌发育过程中,α-黏着斑蛋白的表达分布存在增龄变化;α-黏着斑蛋白的分布方式与闰盘的发育是一致的。

BACKGROUND： Macula adherens protein is found closely associated with congenital cardiac malformation and myocardial differentiation. OBJECTIVE： To investigate the expression characteristics of α-macula adherens protein in rat heart, as well as the property of age-dependant expression during myocardial growth. DESIGN： Randomized controlled, observational comparative study. SETTING： Department of Cell Biology of Xinxiang Medical College; Department of Bioengineering and Agricultural Economics of Puyang Vocational Technical School. MATERIALS： This study was conducted at the Morphological Laboratory of Xinxiang Medical College between January and June 2003. Totally 28 Wistar rats of clean grade were divided into infant group, youth group, middle-age group, and old-age group with 7 rats in each group. METHODS： All rats were anaesthetized and then cardiac tissues were cut into consecutive coronal slices of 5 μm thick. The expression of α-maculaad herens protein in rat myocardium of infant, youth, middle-age and oldage groups was detected using IHC method. The positive cells displayed brownish yellow granules on the surface, cytoplasm and intercalated disc. Routine HE staining was performed on all specimens for structural comparison. MAIN OUTCOME MEASURES： The expression of α-macula adherens protein in rat myocardium of different groups. RESULTS： All the 28 rats entered the final results analysis. ① α-macula adherens protein was found to be expressed in myocardium in atrium, ventricle, papilla muscles and interventricular septum. ② In infant rats, the expression of α-macula adherens protein was mainly observed in intercalated disc at the end of myocardium, with less expression on cell surface and in cytoplasm; in contrast, α-macula adherens protein in young, middleaged and old rats was found to be typically expressed in intercalated disc at the end of myocardium. CONCLUSION： The expression of α-macula adherens protein displays age-dependant manner during rat myocardial development; moreover, the expression of α-macula adherens protein is found to be consistent with the development of intercalated disc.