天年饮延缓亚急性衰老大鼠生殖功能及性腺衰退的作用(英文)

Action of tiannianyin on delaying declined reproductive function and hypogonadism in subacute senile rats

背景:随着机体衰老性腺功能逐渐降低,中国传统医学认为精气虚弱是导致机体衰老的主要原因。目的:观察中药天年饮对D-半乳糖致亚急性衰老大鼠血清睾酮含量、睾丸组织超氧化物歧化酶的活性及睾丸生精细胞增殖细胞核抗原表达的影响。设计:随机对照实验。单位:承德医学院基础医学研究所。材料:实验于2003-04/07在承德医学院基础医学研究所进行。选择成年雄性SD大鼠40只,随机分为4组,每组10只。正常组不做任何处理,模型组、用药组、阴性对照组采用D-半乳糖连续腹腔注射200mg/(kg·d)制备亚急性衰老动物模型,模型组大鼠于造模成功后不再作任何处理。用药组大鼠于造模成功后每天用天年饮灌胃,1次/d,共30d;阴性对照组大鼠在造模成功后每天灌胃同等剂量的生理盐水,时间同用药组大鼠。方法:各组大鼠血清中睾酮的含量采用酶联免疫吸附方法检测,睾丸组织中超氧化物歧化酶的活性采用黄嘌呤氧化酶法检测,增殖细胞核抗原的表达情况采用免疫组织化学法观察。主要观察指标:①血清中睾酮的含量。②睾丸组织中超氧化物歧化酶的活性。③睾丸生精细胞增殖细胞核抗原的表达情况。结果:40只大鼠均进入结果分析。①血清中睾酮的含量:模型组大鼠低于正常组(P<0.05),用药组高于模型组(P<0.05)。②睾丸组织超氧化物歧化酶的活性:模型组明显低于正常组(P<0.01),用药组明显高于模型组(P<0.01)。③睾丸生精细胞增殖细胞核抗原的表达情况:模型组低于正常组(P<0.05);用药组明显高于模型组(P<0.05)。结论:天年饮可延缓生殖功能的退化,具有一定的延缓衰老的作用。

BACKGROUND： Hypogonadism happens gradually with body aging. Traditional Chinese medicine views that deficiency of essential qi is the chief reason for body aging. OBJECTIVE： To observe the influence of tiannianyin, the Chinese herb, on serum testosterone, superoxide dismutase （SOD） activity in testicular tissue and expression of proliferating cell nuclear antigen of sperma togeniccells in testes in subacute senile rats induced by D-galactose. DESIGN： Randomized controlled experiment.SETTING： Basic Medicine Institute of Chengde Medical College. MATERIALS： The experiment was perlormed in Basic Medicine Institute of Chengde Medical College from April to July 2003, in which, 40 adult male SD rats were employed and randomized into 4 groups, 10 rats in each. In normal group, no any management was done. In model group, medication group and negative control, D-galactosie was used for abdominal injection continuously at 200 mg （kg·d）, once a day, totally for 40 days to prepare the model of subacute senile animal. In model group, no any management was done after modeling success. In medication group, gastric infusion with tiannianyin.Tiannianyin was administrated everyday after modeling success, once a day, totally for 30 days. In negative control, gastric infusion with physiological saline of same dosage was administrated after modeling success for same days. METHODS： Enzyme linked immunosorbent assay （ELISA） was used for determination of serum testosterone content, xanthine oxidase assay was used for determination of SOD activity in testicular tissue and immunohistochemical method was used to observe expression of proliferating cell nuclear antigen in rats of every group. MAIN OUTCOME MEASURES： ① serum testosterone content; ② SOD actiyity in testicular tissue; ③ expression of proliferating cell nuclear antigen of spermatogenic cells. RESULTS： Forty rats all entered result analysis. ① Serum testosterone content： That in model group was lower than normal group（P 〈 0.05）, and that in medication group was higher than model group（P 〈 0.05）. ② SOD activity in testicular tissue： That in model group was lower remarkably than normal group（P 〈 0.01）, and that in medication group was higher remarkably than model group（P 〈 0.01）. ③ Expression of proliferating cell nuclear antigen of spermatogenic cells： That in model group was lower than normal group（P 〈 0.05）, and that in medication group was higher remarkably than model group（P 〈 0.05）. CONCLUSION： Tiannianyin delays degeneration of reproductive functionan and aging of certain degree.