参麦注射液对急性缺氧-复氧心肌细胞凋亡的影响

Effect of Shenmai injection on cardiomyocyte apoptosis after acute anoxia-reoxygenation

目的:观察参麦注射液对急性缺氧-复氧后心肌细胞凋亡的影响,并探讨其可能机制。方法:采用原代培养的大鼠心肌细胞,通过化学缺氧法使细胞缺氧5min,再恢复氧供应15min,复制心肌细胞缺氧-复氧(anoxi-a-reoxygenation,A/R)模型。Annexin V-FITC/PI双染色流式细胞仪检测细胞凋亡百分率;Fluo-3负载激光扫描共聚焦显微镜观察细胞内钙离子水平。结果:A/R组心肌细胞凋亡百分率明显高于正常组,细胞内平均钙离子荧光强度也显著强于正常组(P<0·01)。参麦注射液组细胞凋亡率显著小于A/R组,同时细胞内钙超载也明显轻于A/R组(P<0·01)。结论:参麦注射液能有效抑制缺氧-复氧心肌细胞的凋亡,这种保护作用的机制之一可能是通过减轻细胞内Ca2+超负荷实现的。

AIM： To study the effects of Shenmai injection on cardiomyocytes apoptosis after acute anoxia- reoxygenation （A/R） and the possible mechanism. METHODS： In this experiment, cultured cardiomyocytes isolated from neonatal rat were used. Model of myocardial anoxia - reoxygenation injury was produced by depriving oxygen for 5 min and then restoring oxygen for 15 min. The apoptotic cells was detected by flow cytometry to detect labbled Annexin V - F1TC/PI. The intracellular calcium level was observed by laser scanning confocal microscopy markered Fluo- 3/AM. RESULTS： In anoxia- reoxygenation group, the percentage of apoptotic cells and fluorescent intensity of intracellular calcium were both prominently higher than those in control group （P 〈 0.01 ）. The apoptotic rate in Shenmai injection group was notably less than that in A/R group and the intracellular calcium overload was also less obvious in Shenmai injection group than that in A/R group （ P 〈 0.01 ）. CONCLUSION： Shenmai injection has notable effects on attenuating apoptotic rate after acute anoxia - reoxygenation in cardiomyocytes, which may be partly due to its alleviating intracellular calcium overload.