豌豆抑芽基因PSAD1的克隆及植物表达载体的构建

Cloning of Dormancy Gene PSAD1 and Construction of Its Plant Expression Vetor

提取豌豆基因组DNA,用PCR法克隆该基因,连接到pUCM18-T载体上并测定其全序列,测序正确后,将该基因亚克隆到载体PBI121上,并将载体PBI121上的CaMV35S启动子替换为伤诱导启动子,构建成了植物表达载体pPOD,为后续工作打下良好基础。

The function for pisum sativum gene PSAD1 was to restrain the growth of axillary bud. The Pisum sativum genomic DAN was extracted and then the PSAD1 gene was cloned from pea genomic DNA template by PCR and linked to pucml8 -T vector and sequencing. The sequence analysis showed that the sequencing result was exact, and the cloned gene was lined to the pBI121 vector which was to replace the CaMV35S promoter with wound inducible promoter in construction of plant expression vetor pPOD, for providing good foundation for the following work.