组织因子途径抑制物-2影响人前列腺癌细胞侵袭能力的实验研究

The experimental study on the role of tissue factor pathway inhibitor-2 in human prostate cancer invasion

目的:探讨人组织因子途径抑制物2(TFPI2)对基因转染入人前列腺癌细胞株PC3M体外和体内侵袭转移能力的影响。方法:通过Boyden小室体外侵袭转移模型,评价转染成功的PC3MTFPI2细胞和未转染的PC3M迁徙和侵袭能力的强弱;并且分别进行裸鼠接种实验,观察两组间其形成的局部新生物相对体积、组织浸润程度,以及有无远处转移的差异。结果:转染成功的PC3MTFPI2细胞中,证实有TFPI2mRNA和相应蛋白质的表达,转染成功的PC3MTFPI2细胞较未转染的PC3M体外侵袭能力明显下降,接种PC3MTFPI2组细胞的裸鼠较接种未转染的PC3M的裸鼠形成的新生物肌层浸润明显减少。结论:人TFPI2基因真核表达载体成功转染人PC3M并可以在PC3M中得到表达,TFPI2的表达可以抑制PC3M的体外和体内的侵袭能力。

Objective： To investigate the role of TFPI-2 in human prostate cancer cells invasion in vitro and in vivo. Methods：Compared the migration and invasion ability between transgeneic PC3M-TFPI-2 cells and PC3M cells by the Boyden chamber in vitro, and evaluated the difference of the degree of tissue infiltration and tumor metastasis etween PC3M-TFPI-2 group and control group after subcutaneously injected into the nude mice＇s back. Resuits：The stable-transfected cells line PC3M-TFPI-2 can express mRNA and protein of TFPI-2. Compared with control groups the number of TFPI-2-expressing cells（PC3M-TFPI-2） that traversed the Matrigel matrix-coated membrane decreased obviously, the tumors formed by PC3 M-TFPI-2 clone cells were well demarcated, while the control group showed an obscure boundary and invaded into the muscle layer. Conclusions： The expression of TFPI-2 can inhibit the invasive ability of PC3M in vitro and in vivo.