涎腺黏液表皮样癌中p16、p15的表达及相关性研究

The Correlation of p16 and P15 Expressions in Salivary Gland Mucoepidermoid Carcinoma

[目的]研究p16、p15在正常涎腺组织和黏液表皮样癌中的表达,探讨其在黏液表皮样癌发生、发展中的作用及临床意义。[方法]应用免疫组化S-P法检测10例正常涎腺组织、45例黏液表皮样癌中p16、p15蛋白的表达。[结果]黏液表皮样癌中p16、p15的阳性表达率分别为62.2%、73.3%,显著低于其在正常涎腺组织中的表达(100%)(P<0.05),且随肿瘤分化程度的降低而递减并具有显著性差异,其中p16的阳性率与淋巴结转移有关。p16、p15两基因在黏液表皮样癌的表达中呈显著相关性(P<0.05)。[结论]p16、p15的失表达与黏液表皮样癌的发生和发展关系密切,p16基因的异常可能是更为直接的因素。

[Purpose ]To explore the expressions of p16 and p15 in normol salivary gland tissue and in mucoepidermoid carcinoma （MEC）.The role of carcinogenesis and clinical significance are investigated. [Methods]The expression of p16 and p15 protein in 10 cases of normal salivary gland tissue and 45 cases with MEC was detected by the streptavidin-peroxidase （S-P） immunohistochemical method.[Results]The positive rates of p15 and p16 in MEC were 62.2% and 73.3% respectively,which was significantly lower than those in normal salivary gland tissue（100%） （P〈O.05） and the positive rates decreased as the tumor lost its differentiation. The expression of p16 was related to lymph node metastasis. There were significant or correlation between the expression of p16 and p15 in MEC （P〈0.05）. [Conclusions]Lost expression of p15 and p16 are closely correlated with the process of carcinogenesis in MEC, probably the abnormality of the p16 gene is a direct factor.