流式细胞术检测肺癌瘤组织和外周血角蛋白阳性细胞表达

Clinical Significance of the Expression Rates of Cytokeratin Positive Cells in Tumor Tissue and Peripheral Blood Cells from Patients with Lung Carcinoma by Flow Cytometry

[目的]探讨肺癌患者瘤组织和外周血角蛋白(cytokeratin,CK)阳性细胞的表达及其临床意义。[方法]采用流式细胞术检测和分析了43例肺癌患者瘤组织和外周血CK+细胞的表达情况。[结果]肺癌患者瘤组织和外周血CK+细胞检出率均显著高于相应对照组(P<0.01或P<0.05),且两者呈良好的正相关(r=0.325,P<0.05)。肿瘤患者CK+细胞检出率与肿瘤患者性别、年龄无关(P>0.05);而与肿瘤临床分期、病理分级、瘤灶数、肿瘤大小、转移情况以及癌细胞倍体类型、增殖活性和凋亡水平密切相关(P<0.05或P<0.01)。[结论]CK+细胞表达水平与患者临床生物学行为及瘤细胞生物学特性关系十分密切;CK+细胞可以作为肺癌的诊断、恶性度判断、微转移及患者预后估计的标志物。

[Purpose]To investigate the clinical significance of the expression level of cytokeratin positive （CK＋） cells in tumor tissue and peripheral blood from patients with lung carcinoma. [Methods]The expression rates of CK＋ cells in tumor tissue and peripheral blood ceils from 43 patients with lung carcinoma were measured and analyzed by flow cytometry. [Results] The expression rates of CK＋ cells in tumor tissue and peripheral blood cells from patients with lung carcinoma were higher than those from normal control groups （P〈0.01 or P〈0.05）. The expression rates of CK＋ cells between tumor tissue and peripheral blood cells showed satisfactory positive correlation in patients with lung carcinoma （ r=0.325,P〈0.05）.Thes tatistical analysis showed no significant correlation between the expression rates of CK＋ cells and sex, age （P〉0.05）, but closely correlation existed between the expression rates of CK＋ cells and clinical staging, pathological grade, number of tumors , tumor size, metastasis state as well as the type of ploid, proliferation activity and apoptosis level of tumor cells（P〈0.05 or P〈0.01 ）. [Conclusion ]The correlation were very intimate between the expression level of CK＋ cells and clinical behavior as well as biology characteristic of tumor cells in patients with lung carcinoma. The expression rates of CK＋ cells may be regarded as a marker of diagnosis, judgement of malignancy degree, prognosis and micrometastasis in lung carcinoma.