局灶节段性肾小球硬化患儿尿TGF-β1检测的临床意义

Clinical significance of detection of urinary transforming growth factor-β1 in children with focal segmental glomerulosclerosis

目的:评估局灶节段性肾小球硬化(FSGS)患儿尿液中TGF-β1检测临床意义。方法:应用酶联免疫吸附法(ELISA)测定35例FSGS患儿,并对照观察30例系膜增生性肾小球肾炎(MsPGN)、30例轻微病变(ML)和30例对照组儿童尿液中的TGF-β1水平。结果:FSGS组尿液中TGF-β1水平与MsPGN、ML、对照组相比差异有非常显著性(P<0.01)。FSGS中,间质炎症细胞浸润和(或)纤维化组与非炎症细胞浸润和(或)纤维化组相比,TGF-β1水平差异有非常显著性(P<0.01);大量蛋白尿组与非大量蛋白尿组TGF-β1差异亦有显著性(P<0.05)。结论:FSGS患儿的尿液中TGF-β1水平升高,其与间质炎症细胞浸润及蛋白尿严重程度相关。检测尿液中TGF-β1可能是反映肾硬化及病情活动的指标之一。

Objective：To evaluate the clinical significance of detection of urinary transforming growth factor-β1（TGF-β1） in children with focal segmontal glomerulosclerosis（FSGS）.Methods： The uninary TGF-β1 levels were measured in 35 children with FSGS,30 with mesangial proliferative glomerulonphritis（MsPGN）,30 with minor lesion（ML） and 30 healthy children（control） by cnzyme-linked immounosorbent assay（ELISA）.Result：It was indicated that the TGF-β1 level in FSGS group was higher significantly than those in MsPGN,ML and control group（P〈0.01）,In ESGS group,the TGF-β1 levels in subjects with inflammatory cell infiltration and/or fibrosis in interstital tissues and severe protoinuria were significantly different from those without inflammatory cell infiltration and/or fibrosis in intersitial tissues and severe groteinuria（P〈0.01-0.05）,Conclusion：The uninary TGF-β1 level increased in ESGS group and was correlated to inflammatory cell infiltration and/or fibrosis in intersitial tissues and the severity of proteinuria.Detection of uninary TGFβ1 level might indicate the coditions of renal sclerosis.