枸杞对虹膜色素上皮细胞增殖活性干预作用的研究

Research of an effect of Lycium bararum L on the proliferation of iris pigment epithelium

目的寻找能够促进虹膜色素上皮细胞(irispigmentepithelium,IPE)增殖活性的相关因素。方法以激光共聚焦显微镜为研究手段,分析枸杞水煎液作用下,IPE线粒体膜电位(mitochondrialmembranepotential,MMP)的变化。通过MTT检测方法了解枸杞对IPE增殖的影响。结果枸杞水煎液在3.125mg/ml到50mg/ml范围内对IPE具有明显的促增殖作用,与剂量呈现相关性。枸杞使IPE细胞线粒体膜电位明显上升,浓度达到22mg/ml时细胞线粒体膜电位明显升高,后逐渐稳定。浓度达到54mg/ml时曲线再次上升。结论枸杞水煎液具有较好的IPE活性保护作用,对体外培养的IPE具有良好的促增殖作用。

OBJECTIVE To investigate the effect of Lycimn bararum L on the proliferation of iris pigment epithelimn （IPE） .METHODS The mitochondrial membrane potential （MMP） of IPE treated with Lycimn bararum L was determined by laser scan confocal microscopy. Using the technologies of MTT, we investigated the effect of this drug on the proliferation of IPE. RESULTS Lycimn bararum L could significantly increased the mitochondrial metabolic ability of IPE and increased the MMP of IPE. CONCLUSIONS Some Chinese herbs such as Lycimn baranum L could protect IPE effectively and promote proliferation of IPE in culture.