舒肝明目丸对兔视神经损伤的保护作用

An experimental study of the protective effect of shuganmingmu(舒肝明目)pill on injury of optic nerve in rabbit by clipping

目的观察舒肝明目丸对视神经损伤的保护作用。方法24只兔随机分为正常对照组、手术创伤对照组、模型对照组、药物治疗组。手术创伤组只分离球结膜及剪断直肌,封闭创口。模型组及药物组除按手术创伤处理外,用1024g压力的钳子于12点位球后2～3mm处夹紧视神经15秒造成视神经夹伤后缝合创口。将模型对照组及药物治疗组再分为3天组及14天组。共分6组,每组4只兔,观察各组在夹伤后不同时间段的视网膜神经节细胞及视网膜厚度、视网膜丙二醛(malondialdehyde,MDA)含量及超氧化物歧化酶(superoxidedismutase,SOD)活性。结果在以上指标改善方面,药物组优于模型组(P<0.01),手术创伤组与正常对照组差异无显著性(P>0.05)。结论舒肝明目丸对兔夹伤型视神经损伤具有保护作用。

OBJECTIVE To study the protective effect of shuganmingmu（舒肝明目） pill on injury of optic nerve.METHODS Twenty - four rabbits were divided randomly into nomal group（NG） ,the operational wound group（OWG）, the model group （MG）, the treated group （TG） .The OWG was only separated from conjtmctiva and cut rectors then sutured except for the treatment of operational wound the optic nerve behind eyeball 2-3mm in the MG and TG were dipped tightly and caused injured in 1024g pressure forceps then sutured. Moreover the MG and TG were divided into 3 days group and 14 days group. There were 4 rats each group in total 6 groups. To observe the retinal ganglion cells （RGLs）, the thickness of retina （TRL）, the contents of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） were determined in various periods after clipping.RESULTS The improvement of above index the TG was better than the MG （P〈0.01 ）, while there was not significant difference between the OWG and NG （P〉0.05） . CONCLUSIONS Shuganmingmu pill could be effective in the protection of injury of optic nerve.