盐酸氨溴索对胎鼠肺表面活性物质的影响

Effect of ambroxol on the pulmonary surfactant in fetal rat lung

目的:探讨盐酸氨溴索(沐舒坦)对胎鼠肺表面活性物质合成的影响。方法:选择健康清洁级妊娠的SD大鼠18只,随机分为3组,沐舒坦治疗组6只,地塞米松治疗组6只,对照组6只。各组大鼠于妊娠第16、17、18天尾静脉分别注射沐舒坦75mg/kg、地塞米松0.8mg/kg、生理盐水2ml/只。于妊娠第19天剖宫产,取出胎仔肺测定磷脂含量(肺表面活性物质主要成分为磷脂)。结果:沐舒坦治疗组肺磷脂含量为(0.72±0.06)mmol/g湿肺,地塞米松治疗组为(0.73±0.19)mmol/g湿肺,对照组为(0.62±0.09)mmol/g湿肺,沐舒坦治疗组、地塞米松治疗组与对照组差异有显著性(P<0.01),沐舒坦治疗组与地塞米松治疗组之间差异无显著性(P>0.05)。结论:沐舒坦能促进胎鼠肺表面活性物质合成,且与地塞米松作用效果相似,有可能成为临床上促胎肺成熟的药物。

Objective： To evaluate the effect of Ambroxol on the synthesis of pulmonary surfactant in fetal rat. Methods： Eighteen SD pregnant clean rats were divided into three groups randomly. Ambroxol group, Dexamethasone group and control group were given Ambroxol 75 mg/kg, Dexamethasone 0.8 mg/kg and saline 2 ml respectively, which were injected through the caudal vein on the 16th, 17th, and 18th gestational day. On the 19th gestatlonal day, cesareans were carried out and phosphollpids in fetal rat lung were measured （The main compositions of pulmonary surfactant was phosphollpids）. Results： The concentration of phosphollplds in wet lung were （0.72 ± 0.06） mmol/g and （0.73 ± 0.19） mmol/g in Ambroxol group and Dexamethasone group respectlvely, while it was （0.72 ± 0.06） mmol/g in control group. Both Ambroxol group and Dexamethasone group had significant difference compared with control group（P〈0.01）, but there was no difference compared between the Ambroxol group and Dexamethasone group（P〉 0.05）. Conclusion： Ambroxol can increase the levels of pulmonary surfactant in fetal rat, is similar to Dexamethasone. It suggests Ambroxol be drug to promote fetal lung maturing.