利多卡因对哮喘大鼠肺泡Ⅱ型上皮细胞影响的实验观察

Experinental study on the effect of lidocaine on alveolar type Ⅱ epithelial cells in asthnatic rats.

目的:探讨利多卡因对哮喘大鼠肺泡Ⅱ型上皮细胞(AT-Ⅱ)的影响。方法:用卵白蛋白(OVA)制备wistar大鼠哮喘模型并用利多卡因雾化吸入进行干预。采用硝酸还原酶法测定支气管肺泡灌洗液(BALF)中一氧化氮(No)浓度;分离和纯化AT-Ⅱ,利用吖啶橙(AO)荧光染色法检测AT-Ⅱ细胞的损伤率, 并与NO浓度进行相关分析。结果:哮喘组BALF中NO浓度显著高于正常对照组(P<0.01)和利多卡因组(P<0.05);哮喘组AT-Ⅱ细胞损伤率分别高于正常对照组(P<0.01)和利多卡因组(P<0.05);BALF中NO浓度与AT-Ⅱ细胞损伤率呈显著正相关(r=0.903,P<0.01)。结论:哮喘时AT-Ⅱ细胞损伤增加与体内NO的合成和释放增多有关,而利多卡因有降低BALF中NO水平及AT-Ⅱ细胞保护作用。

Objective： To study the effect of lidocaine on alveolar type Ⅱ epithelial cells in asthmatic rats. Methods： In the experiment,the Wistar rat model of asthma was established by the ovalbumin （OVA） challenge methods and the intervention of lidocaine was performed. The concentrations of NO in bronchoalveolar lavage fluid（BALF） were measured with nitrate reductase technique and the injury rates of AT-Ⅱ cells were detected with acridine orange（AO） fluorescent staining after isolated and purified. In addition,the correlation analysis between the injury rates of AT-Ⅱ cells and the concentrations of NO in BALF was performed. Results： Respectively, the concentrations of NO in BALF of the asthma group were higher than that of the control group（P〈 0.01） or the lidocaine-treated group（P〈 0.05）. The indury rates of AT-Ⅱ cells in the asthma group were higher than that in the control group （P〈0.01） or lidocaine-treated group （P〈0.05）. There was a significantly positive correlation between the concentrations of NO in BALF and the indury rates of AT-Ⅱ cells [（r=0.903, P〈 0.01）l. Conclusion： The injury of AT-Ⅱ cells of asthmatic rats is significant and its mechanisms is partly related to the increased production and release of NO in vivo. Lidocaine can decrease the concentrations of NO in BALF and have cytoprotection of AT- Ⅱ cells.