IL-4和IL-13在溃疡性结肠炎中的表达

Expression of Interleukin-4 and Interleukin-13 in ulcerative colitis

目的探讨Th2类细胞因子IL4和IL13在溃疡性结肠炎中的作用。方法收集30例经内镜检查证实的UC患者结肠黏膜和血清标本,20例性别、年龄相匹配的同期大肠息肉患者(取其正常组织)作为对照组。用RTPCR法和酶联免疫吸附法(ELISA)检测IL4及IL13的mRNA表达及其血清含量。结果轻度UC患者IL4和IL13的mRNA表达与对照组比较无统计学意义(P>0.05),中重度UC患者IL4和IL13的肠黏膜表达明显下降(P<0.05)与对照组比较,二者血清含量未见明显区别(P>0.05)。结论IL4和IL13在UC的发病中起重要作用。

Objective Since the roles ofinterleukin （IL-）4 and IL-13 remains unclear, this study aimed at studying the expression of interleukin （IL）-4 and IL-13 in ulcerative colitis （UC）.Methods In colonic biopsies and peripheral blood from 30 UC and 20 normal controls , mRNA expressions of IL-4 and IL-13 were assessed by reverse transcription-polymerase chain reaction（RT-PCR）, and levels of IL-4 and IL-13 in serum were measured using ELISA. Results Our results showed that the mRNA expression of IL-4 and IL-13 were significantly reduced in UC patients when compared with normal controls （P〈0. 05） . No differences could be detected of IL-4 and IL-13 levels in serum as compared between patients with UC and control. Conclusion The severity of UC is associated with decreased colonic IL-4 and IL-13 suggesting that IL-4 and IL-13 may play important roles in the pat hogenesis of UC.