摘要
目的:观察亚硒酸钠对大鼠肾小球系膜细胞(RGMC)表达环氧化酶-2(COX-2)的影响,从而研究硒在抗糖尿病肾病中的作用机制。方法:分别以高葡萄糖、糖基化终产物,高胰岛素和过氧化氢孵育RGMC;先给予亚硒酸钠预处理细胞后,再分别给予上述4种因素孵育RGMC,观察细胞COX-2 mRNA的表达。结果:4种刺激因素均可作为独立因素,导致RGMC COX-2 mRNA表达量的增加;亚硒酸钠能抑制上述4种因素所致的COX-2 mRNA的表达。结论:亚硒酸钠通过抑制COX-2 mRNA在RGMC中的表达,从而有效地防治糖尿病肾病的发生发展。
Objective ：To observe the effect of sodlium selenite on expression of Cyclooxygenase - 2 （COX-2 ） mRNA in rat mesangial cells （RGMC）, and to study the mechanism of ,sodium selenite in anti diabetic nephropathy. Methods ：RGMC was incubated with high glucose, advanced glycosylation end products （AGE）, high insulin and H2O2 respectively; simultaneously, RC-MC pre- treated with sodium selenite was incubated with above factors. The expre,ssion of COX- 2 mRNA was detected and compared. Results：The four factors increased the expre,ssion of COX-2 mRNA independently;sodium selenite inhibited the expression of COX-2 mRNA by the four factors distinctly. Conclusion ：Sodium selenite suppresses the expression of COX-2 mRNA in RGMC, which suggests that sodium selenite may play a significant role in the prevention of diabetic nephropathy by inhibition of the expression of COX-2 mRNA in RGMC.
出处
《重庆医科大学学报》
CAS
CSCD
2005年第4期493-496,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(NO.30370670)
