新鲜羊膜与保存羊膜中HGF、bFGF表达的对照研究

Comparative study on the expressions of HGF and bFGF between fresh and preserved amniotic membranes

目的:研究肝细胞生长因子(HGF)、碱性成纤维细胞生长因子(bFGF)在新鲜和两种保存羊膜中的表达,探讨新鲜和保存羊膜的生物学活性差异。方法:取健康剖宫产产妇胎盘羊膜,随机分成三组:新鲜组、DMEM/甘油-80℃保存组、纯甘油4℃保存组(保存1、3个月),应用免疫组织化学方法,检测HGF、bFGF在各组羊膜中的表达。结果:HGF、bFGF主要分布于羊膜上皮层。HGF在DMEM/甘油组和纯甘油组保存1、3个月时的表达明显低于新鲜羊膜组,统计学上差异有显著性(P<0.0001),bFGF在两组保存3个月时的表达明显低于新鲜羊膜组,统计学上差异有显著性(P<0.01)。结论:羊膜经DMEM/甘油-80℃、纯甘油4℃保存后,HGF、bFGF减少,并随保存时间的延长活性逐渐下降。

Objective ：To study the expressions of hepatocyte growth factor （HGF） and basic fibroblast growth factor （bFGF） in fresh and two preserved amniotic membranes （AM）, and to explore their biological vitality. Methods：The amniotic membranes from the healthy puerperia with abdominal delivery were randomly divided into three groups： fresh group, DMEM/glycerol preserved group at -80℃ and glycerol preserved group at 4℃ （for 1 and 3 months）. [mmunohistochemical method was used to investigate the changes in the expressions of HGF and bFGF among the three groups. Results ： HGF and bFGF were distributed in the epithelium of AMs mostly. The expressions of HGF in both preserved AMs for 1 and 3 months decreased significantly compared with fresh AM （P〈 0. 0001 ）, and the expressions of bFGF in both preserved AMs for 3 months decreased significantly compared with fresh AM （P 〈0.01 ）. Conclusion ： HGF and bFGF both decrease in AMs preserved in DMEM/glycerol at - 80℃ and in glycerol at 4℃, and their vitality declines gradually with preserving time.