摘要
在酸性介质中,钼酸根、钨酸根和偏钒酸根等同多酸根与牛血清白蛋白(BSA)和人血清白蛋白(HSA)等蛋白质反应形成结合产物时,会导致溶液的共振瑞利散射(RRS)急剧增强,最大散射峰均位于470 nm.反应的适宜酸度分别为pH 0.9～1.3(钼酸根-BSA体系),pH 1.1～1.4(钨酸根-BSA体系)和pH 0.6～0.9(偏钒酸根-BSA体系).在一定的浓度范围内,不同的反应体系RRS强度与蛋白质浓度成正比,均可用于蛋白质的测定.反应具有很高的灵敏度,不同的同多酸对BSA的检出限(3σ/s)介于4.1～30.5 μg/L之间,其中钼酸根体系的灵敏度最高.考察了共存物质的影响并研究了方法的分析应用.
In an acid solution, an isopoly-acid anion such as isopoly-molybdic acid, isopoly-tungstic acid or isopoly-vanadic acid reacts with a protein such as bovine serum albumin （BSA） and human serum albumin（HSA） to form a binding product. It results in producing of a strong resonance Rayleigh scattering （RRS）and appearance of a new RRS spectrum with the maximum scattering wavelength of 470 nm. The optimum pH ranges are 0.9 - 1.3 for isopoly-molybdic acid-BSA system, 1.1 - 1.4 for isopoly-vanadic acid-BSA system and 0.6 - 0.9 for isopoly-tungstic acid-BSA system, respectively. The intensity of RRS is directly proportional to the concentration of protein in a certain range depending on the different reaction system. The reactions wit hhigh sensitivities have the detection limits （3σ/s） for BSA between 4.1 and 30.5 μg/L depending on the different isopoly-acid used. The intensity of RRS was enhanced along with the molecular weights of the isopoly acids. The isopoly-molybdic acid system has the highest sensitivity. RRS can be used as a means to study the interaction of colorless and nonfluorescence small molecule with protein and as a method for the quantitative determination of trace amount of protein.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第8期1125-1128,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目(No.20175018)
