川芎嗪和黄芪对尾部悬吊大鼠比目鱼肌肌球蛋白ATP酶活性及肌萎缩的影响

Effects of Ligustrazine and Radix Astragali on Activities of Myosin Adenosine Triphosphatase of Soleus Muscle and Muscle Atrophy in Tail-suspended Rats.

目的探讨川芎嗪和黄芪对吊尾大鼠比目鱼肌(SOL)肌球蛋白ATP酶(mATPase)活性及肌萎缩的影响。方法尾部悬吊法建立模拟失重模型,Ca2+-ATPase法测定SOL的mATPase活性。结果1)与吊尾组(TS)相比,在腹腔注射川芎嗪组和黄芪组,SOL中Ⅱ型肌纤维比例均显著降低,且黄芪组Ⅰ、Ⅱ型肌纤维的比例与同步饲养组相比亦无显著差异;2)川芎嗪组SOL中Ⅰ型肌纤维横截面积(CSA)显著增大,与同步饲养组相比无显著性差异,黄芪组Ⅰ、Ⅱ型肌纤维CSA及平均CSA均显著地增大,且与同步饲养组相比亦无显著性差异;3)川芎嗪组和黄芪组梭内肌各纤维的mATPase染色均与同步饲养组相近。结论川芎嗪和黄芪对大鼠SOL的失重性肌萎缩及慢肌向快肌的转化均有明显的对抗作用,且均可显著抑制模拟失重导致的梭内肌各纤维mATPase活性的升高。

Objective To study the effects of Ligustrazine （Lig） and Radix Astragali （Rad） on activities of myosin adenosine triphosphatase （mATPase） of soleus muscle and muscle atrophy in tail-suspended rat. Method Weightlessness was simulated by tail suspension in female rats. The activities of mATPase of intrafusal and extrafusal fibres in soleus muscle were detected by method of Ca^2＋-ATPase. Result 1） Compared with tail-suspended（TS） group, the percentage of type Ⅱ fibres of SOL in both Ligustrazine（Lig） group and Rad group decreased distinctly. Furthermore, the percentage of type Ⅰ and type Ⅱ fibres of SOL in Rad group showed no difference with control （CON）group. 2） The type Ⅰ CSA of Lig group was markedly larger than that in TS group, and there was no difference as compared with that of CON group. CSA of type Ⅰ ,Ⅱ and average CSA of Rad group were remarkably larger than those in TS group, and there were no difference as compared with those of CON group. 3） mATPase activities of intrafusal fibres in both Lig group and Rad group were approximate to that of CON group. Conclusion Lig and Rad are both able to effectively prevent muscle atrophy caused by tail suspension, restrain the slow-twitch muscle transform to fast-twitch muscle and control the increase of mATPase activities caused by weightlessness.