重症监护病房耐甲氧西林金黄色葡萄球菌及其肠毒素的检测和耐药性分析

Analysis of methicllin resistant Staphylococcus aureus in ICU and the detection of enterotoxin in ICU

目的评价乳胶结合实验检测重症监护病房(ICU)耐甲氧西林金葡菌(M RSA)及金葡菌肠毒素,并进行耐药性分析。方法收集130株金葡菌临床分离株,通过药敏试验将其分为耐甲氧西林金葡菌和甲氧西林敏感金葡菌(M SSA),用反向间接血凝试验(RPHA)检测金葡菌肠毒素。结果M RSA产肠毒素为67株,M SSA产肠毒素为19株,M RSA产肠毒素率为100%,M SSA产肠毒素率为30%。结论重症监护病房应重视M RSA的检测和金葡菌肠毒素的检测。合理使用广谱抗生素。

Objective To evaluate the latex fixation test for detection and survey the prevalence of enterotoxin in ICU as well as to analyze the resistance of methicillin resistant Staphylococcus aureus （MRSA）.Methods Based on the antimicrobial susceptibility test , compared with that of latex fixation test, the 130 clinically isolated cultures were classified into MRSA and methicillin sensitive Staphylococcus aureus （MSSA）. The enterotoxin produced by the strains was detected by reverse passive hemagglutination （RPHA） test. Results The enterotoxins were produced in 67 MRSA with prevalence rate of 100%, while 19 MSSA of 30%. Conclusions The importance to detect the prevalence of MRSAand the enterotoxin produced in ICUand the rational use of broad spectrum antibiotics should be critically valued.