肠上皮内淋巴细胞促L929细胞增殖活性的研究

Effects of conditioned supernatants from intestinal intraepithelial lymphocytes on the proliferation of L929 cell line in mice with stress ulcer

目的:了解应激性溃疡模型中肠上皮内淋巴细胞(iIEL)培养上清促L929细胞增殖活性的变化。方法:采用水浸限制刺激法制备小鼠应激性溃疡模型。分别提取iIEL和脾T淋巴细胞,在含ConA的培养液中培养。采用MTT法检测iIEL和脾T淋巴细胞培养上清的促L929细胞增殖活性。结果:正常鼠iIEL培养上清促L929细胞增殖活性显著高于脾T细胞培养上清(P<0.05)。应激性溃疡发生后,iIEL培养上清促L929细胞增殖活性显著下降,于第4天降至最低(P<0.05),1周后恢复正常。结论:iIEL培养上清中可能含有高水平的促进L929细胞增殖的因子,这种因子的活性在应激性溃疡中可能发生变化。

Objective： To invcstigate the effects of conditioned supernatants from intestinal intraepithelial lymphocytes （iIEL） on the proliferation of L929 cell line in mice with stress ulcer. Methods： Mouse models of stress ulcer were made by using water-immersion restraint stress, iIEL and splenic T lymphocytes were isolated and cultured by ConA， Conditioned supematants were obtained from the cultures of ilEL. The proliferation rate of L929 cells was measured by MTT method. Results： The proliferation rate of L929 cell in the iIEL-conditioned culture medium was significantly higher than that in the splenic T cell-conditioned cuhure medium in the normal control mice （ P 〈 0.05 ）. The promoting effect of ilEL-conditioned supernatants on the proliferation of L929 cell decreased after stress ulcer was induced, reaching the lowest level on the fourth day （ P 〈 0.05 ） , and returned to normal a week later. Conclusion： The conditioned supematants of iIEL may contain factors which may have positive effect on the proliferation of L929 cell line, and the activity of these factors may change in the stress ulcer.