肝硬化大鼠模型脑组织形态变化及机制

Histopathological changes of the brain and its mechanism in liver cirrhosis model of rats

目的:探讨肝硬化时脑组织形态改变及其发生机制。方法:应用计算机扫描技术对16只应用四氯化碳腹腔注射12周制备肝硬化模型的大鼠和9只正常大鼠的大脑皮层的厚度进行计算,同时应用TUNEL方法和苏木素复染后计数大鼠脑细胞凋亡情况。结果:肝硬化动物模型的血氨为(231.80±24.17)μmmol/L,明显高于正常大鼠(48.7±5.87)μmmol/L。肝硬化动物模型大脑皮质的厚度为(215.03±14.94)μm,正常大鼠大脑皮质厚度为(248.35±19.82)μm,P<0.01;脑细胞凋亡指数(0.29±0.11)较正常大鼠(0.03±0.02)明显增高,P<0.05。脑细胞凋亡指数与血氨的水平呈正相关。结论:氨可能诱发肝硬化大鼠脑发生形态变化,脑神经细胞凋亡可能是这种变化的基础。

Objective： To study the histopathological changes of the brain and its mechanism in liver cirrhosis model of rats， Methods： The liver cirrhosis models of rats were made by injecting carbon tetrachloride into tile abdominal cavity for 12 weeks. The thickness of the cortex was measured by computer scanning in 16 liver cirrhosis models of rats and 9 normal rats. The apoptosis of brain cells in liver cirrhosis models was detected by TUNEL staining. Results： The level of blood ammonia in liver cirrhosis models of rats was much higher than that in normal rats [231.80 ± 24.17 （SD） μmmol/L vs. 48.70± 5.87μmmol/L, P 〈0.05 ]. The thickness of cortex in liver cirrhosis models of rats was lower than those in normal rats （215.03± 14.94μm vs. 248.35±19.82μm, P 〈0.01）. The apoptosis index of brain ceils in liver cirrhosis models of rats was larger than that in normal rats （0.29± 0.11 vs. 0.03 ± 0.02, P 〈0.05）. The apoptosis index of brain cells correlated positively to the level of blood ammonia. Conclusion： Ammonia may induce the histopathological changes of the brain in rats with liver cirrhosis and the apoptosis of brain eells may be the foundation of these changes。