PCR法制备乙肝病毒基因芯片目标序列的探讨
被引量：2
摘要
比较对称与不对称PCR法制备的乙肝病毒基因芯片目标序列的杂交效果,并对不对称PCR法中上、下游引物浓度比例的最佳条件进行了探讨。
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第4期318-319,共2页
Journal of China Medical University
基金
辽宁省教育厅科研基金资助项目(20201100)
参考文献4
-
1Bednarczuk T A , R C Wiggins, C W Konat . Generation of high efficiency, single - stranded DNA hybridization probes by PCR[J].Biatechniques, 1991 , 10(4) :478.
-
2Wang E, Miller LD,Ohnmacht GA, et al, High-fidelity mRNA amplification for gene profiling[J].Nat Biotechnol,2000,18( 4 ) :457- 459.
-
3Van Gehter RN,von Zastrow ME,Yool A, et al. Amplified RNA synthesized from limited quantities of heterogenenous cDNA[J].Proc Natl Acad Sci USA, 1990,87 (5) :1663 - 1667.
-
4Ebervdne J,Yeh H, Miyashiro K,et al..Analysis of gene expression in single live neurons[J]. Proe Natl Acad Sci USA, 1992,89 (7) :3010 -3014.
同被引文献31
-
1高宏伟,马永和,段铭,彭龙.应用基因芯片对4种产毒霉菌的检测[J].中国兽医学报,2005,25(4):376-377. 被引量：5
-
2Iwahashi H, Kitagawa E, Suzuki Y, et al. Evaluation of toxicity of the mycotoxin citrinin using yeast ORF DNA microarray and Oligo DNA microarray. BMC Genomics,2007,8:95.
-
3胡娜.基因芯片技术在黄曲霉毒素生物合成相关基因检测中的应用研究.南昌:南昌大学,2006.
-
4van't Veer LJ, Dai H, van de Vijver MJ, et al. Gene expression profiling predicts clinical outcome of breast cancer. Nature, 2002,415 : 530-536.
-
5Chen JJ, Wu R, Yang PC, et al. Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection. Genomics, 1998,51:313-324.
-
6Lien YC, Noel T, Liu H, et al. Phospholipase C-deltal is a critical target for tumor necrosis factor receptor-mediated protection against adriamycin-induced cardiac injury. Cancer Res, 2006, 66: 4329-4338.
-
7Tan J, Zhuang L, Jiang X, et al. Apoptosis signal-regulating kinase 1 is a direct target of E2F1 and contributes to histone deacetylase inhibitor-induced apoptosis through positive feedback regulation of E2F1 apoptotic activity. J Biol Chem,2006,281:10508-10515.
-
8Chaudhry MA. Bystander effect : biological endpoints and microarray analysis ( Review ) . Mutat Res, 2006, 597 ( 1/2 ) : 984112.
-
9Chang PK, Wilkinson JR, Horn BW, et al. Genes differentially expressed by Aspergillus flavus strains after loss of aflatoxin production by serial transfers. Appl Microbiol Biotechnol, 2007, 77:917-925.
-
10Schmidt-Heydt M, Geisen R. A microarray for monitoring the production of mycotoxins in food. Int J Food Microbiol,2007,117 : 131-140.
-
1周立新,袁建成,肖光夏.影响地高辛标记TNF cDNA探针原位杂交效果的因素[J].第三军医大学学报,1995,17(4):338-340. 被引量：2
-
2季士治,雷霁霖,王伟继,孔杰.双列杂交法分析2个大菱鲆养殖群体的杂交效果[J].中国水产科学,2006,13(6):1001-1005. 被引量：15
-
3黄海,马文丽,王洪敏,肖维威,王艳,郑文岭.用巢式PCR方法制备基因芯片的探针[J].生命科学研究,2003,7(3):271-274. 被引量：3
-
4王子慧,于鼎,王瑞绵.PCR引物法及随机引物法标记cDNA探针杂交效率的研究[J].湖北医科大学学报,2000,21(1):4-6. 被引量：5
-
5龚蕾蕾,沈爱国,严美娟,何江虹,顾建新.正、反义β-1,4半乳糖基转移酶Ⅱ和Ⅴ地高辛标记RNA探针的制备和应用[J].交通医学,2003,17(5):593-593.
-
6胡文辉,舒理慧,宋运淳,祝莉莉,何光存.基因组原位杂交鉴定栽培稻与广西药用野生稻杂交后代染色体构成[J].武汉植物学研究,2000,18(2):81-84. 被引量：6
-
7Mefti Korteby Hakima,Kaidi Rachid,Sid Sihem,Boukhelifa Ahmed,Derradji Billel,Kenchache Youce,Mareche Hachemi.Genetical Crossbreeding Effect on the Zootechnical Performances of the Domestic Rabbit （Algeria） x Californian[J].Journal of Life Sciences,2013,7(2):165-170.
-
8武晓敏,黄静,魏燕燕,陈晶,肖培伦,冯达云,武胜昔,刘娟.小鼠Brd2基因探针的制备及其在荧光原位杂交实验中的应用[J].神经解剖学杂志,2014,30(3):335-339. 被引量：1
-
9黄浩杰,张锡然,陈宜峰,崔英霞.应用G显带染色体荧光原位杂交（FISH）技术研究复杂的染色体易位[J].Acta Genetica Sinica,1996,23(5):338-342. 被引量：18
-
10张晶,陈修栋,王善强,周鑫,郑振宇.河南地方野生小鼠与近交系小鼠的扩增片段长度多态性分析[J].中国实验动物学报,2011,19(3):224-227.
