金刚石涂膜材料对成骨细胞骨钙素表达的影响

Effect of the chemical-vapor-deposited diamond film materials on expression of osteocalcin mRNA of osteoblast

目的:探讨不同种植材料表面成骨细胞的功能状态。方法:用Wistar大鼠颅顶骨建立体外培养成骨细胞模型,将第三代成骨细胞接种到金刚石涂膜(CVD)、羟基磷灰石(HA)、纯钛(Ti)3种种植材料表面,通过反转录聚合酶链式扩增方法测定成骨细胞骨钙素mRNA。结果:CVD表面的成骨细胞骨钙素mRNA表达比HA出现早,Ti表面未见骨钙素mRNA表达。结论:CVD表面骨钙素的早期表达,推测其有成骨功能,适合成骨细胞生长。

Objective： To evaluate the cell function of osteoblasts on the surface of different implant denture materials. Methods： The cultured osteohlastic cell model in vitro was made with calvaria of new born Wistar rats. The third generation osteoblasts were plated on the surfaces of 3 kinds of implant materials of chemical-vapor-deposited diamond film （CVD） , hydroxyapatite, and pure titanium. The expressions of osteoealein mRNA were examined by using RT-PCR. Restilts ： The osteocalcin mRNA expression of CVD material group was earlier than that of hydroxyapatite group, and there was no expression in pure titanium group. Conclusion： CVD materials are fit for growth of osteoblasts, and it could promote the formation of the new bone。