急性冠状动脉综合征患者外周血单核细胞核因子κB活性的变化及其意义

Measurement and clinical significance of the activity of nuclear factor B in peripheral blood mononuclear cells in the patients with acute coronary syndrome

目的探讨核因子κB(NF-κB)在急性冠状动脉综合征(ACS)发病中的作用。方法采用ELISA法检测ACS患者63例、稳定型心绞痛患者38例及正常人20例外周血单核细胞NF-κB的活性,并检测其血清C反应蛋白的浓度。结果ACS患者外周血单核细胞NF-κB活性及血清C反应蛋白浓度明显高于稳定型心绞痛患者和正常人(P<0.01),其中急性心肌梗死患者最高;且两者之间呈明显的正相关性。结论ACS血单核细胞NF-κB活性明显升高,促发急性炎症反应,引起斑块的不稳定,在ACS发生中起着重要作用。

Objective To investigate the role of nuclear factor κB activation in acute coronary syndrome（ACS）. Methods 63 patients with ACS（acute myocardial infarction, n = 29;unstable angina pectoris, n =34）, 38 cases with stable angina pectoris and 20 normal controls were enrolled. NF -κB activation in peripheral blood mononuclear cells was detemined by ELISA with the TnmsAM NF -κB p65 Kit, and serum C reactive protein （sCRP） concentration was determined. Results The activity of NF -κB in circulating white cells and sCRP concentration in the patients with ACS were significantly higher than those in SAP and normal controls（ P 〈0.01 ）, especially in AMI.There were positive relationship between the activity of NF - κB and sCRP concentration in the patients with ACS.Conclusion Nuclear factor-κB is markedly activated in patients with ACS, which may be mechanistically involved in the plaque disruption by predisposing acute inflammation reaction, and plays an important role in the pathogenesis of ACS.