松阿扁叶蜂对不同树种挥发物的触角电位反应

Electroantennogram responses of Acantholyda posticalis Matsumura to volatiles of different trees

用MS302生物信号记录分析系统,以顶端记录法测定松阿扁叶蜂成虫对不同树种挥发性物质的EAG反应。结果显示,该蜂成虫对其嗜好的油松、赤松、华山松挥发物的EAG反应敏感,雌成虫放电频率分别为45.2次/s、42.1次/s、42.3次/s,电位值依次为1.022mV、1.139mV、0.706mV,但对其非寄主树种的侧柏挥发物反应微弱,对空气无反应。雌成虫对油松挥发物的不同气流量1.7mL/s、17mL/s、85mL/s的放电频率和电位值分别为24.8次/s、45.2次/s、46.6次/s和0.533mV、1.022mV、1.316mV,表明EAG反应程度因挥发物气流量浓度不同而变化。触角电位与选择性行为的测试结果基本一致,表明松阿扁叶蜂在寄主选择过程中,触角的嗅觉功能起着重要作用。

With the MS302 analysis system for biological signals, the EAG response of Acantholyda posticalis Matsumura adult to volatiles of different tree species was assayed. The results showed that female A. posticalis adult was sensitive to Pinus tabulaeforrnis, P. densiflora and P. armandi, which it was addicted to. To volatiles of Pinus tabulaeforrnis, P.densiflora and P. armandi, the electrofrequency of the female adult was 45.2, 42.1 and 42.3 times per second,respectively and its electrovoltage was 1.022 mV, 1. 139 mV and 0.706 mV, respectively. There was faint EAG response to the non-host tree specie Platycladus orientalis, and no response to the air. The electrofrequency of female adult to different flux （1.7 mL/s, 17 mL/s, 85 mL/s） of the volatiles of P. tabulaeforrnis was 24.8, 45.2 and 46.6 times per second, respectively ; its electrovoltage was 0. 533 mV, 1.022 mV and 1. 316 mV, respectively. This indicated that the EAG response changed with the different flux of volatiles.