不同生物型烟粉虱体内Wolbachia共生菌的检测及其系统树分析

Detection and phylogenetic analysis of Wolbachia in different Bemisia tabaci biotypes

Wolbachia是广泛分布于节肢动物体内一类共生细菌,它能够通过多种机制调控宿主的生殖方式。近年来的研究表明,Wolbachia与许多外来生物的成功入侵相关。本文利用长PCR的方法特异扩增了不同生物型烟粉虱(共24个种群)体内Wolbachia的wsp基因,结果发现B型和Q型烟粉虱入侵种群体内均未检测到Wolbachia,而在非B/Q型的浙江种群和肯尼亚种群体内检测到了Wolbachia。对该wsp基因进行测序并和已知序列进行同源性分析发现,浙江烟粉虱种群的Wolbachia属于B组Con/Rug亚种群,而肯尼亚种群属于B组Btab1亚种群。Wolbachia的存在与否可能与烟粉虱的成功入侵有一定的关系。

Wolbachia is a maternally inherited bacterium that may manipulate the reproduction of its arthropod hosts.Recent research showed that Wolbachia may have contributed to the success of invasive populations. We reported the survey for the presence of Wolbachia endosymbionts in Bemisia tabaci biotypes （24 different populations） using Long PCR method to amplify the wsp gene. The results showed that there were no Wolbachia in both invasive B. tabaci B biotype and Q biotype, while a strain of Wolbachia was detected in the non-B/Q biotype of B. tabaci both from Zhejiang province and Kenya. Sequencing and phylogenetic analysis showed that the strain infesting B. tabaci from Zhejiang province belonged to Con/Rug subgroup of Wolbachia B group, and the strain infesting B. tabaci from Kenya belonged to Btabl subgroup of Wolbachia B group. The presence or abscence of Wolbachia may be related to successful invasion of B. tabaci.