转sck+cry1Ac基因水稻对二化螟及二化螟绒茧蜂存活和生长发育的影响

Effect of transgenic sck+cry1Ac rice on the survival and growth of Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and its parasitoid Apanteles chilonis (Munakata) (Hymenoptera: Braconidae)

在实验室研究了转sck+cry1Ac基因水稻(MSB)对二化螟Chilosuppressalis(Walker)生长、存活以及经寄主对二化螟绒茧蜂Apanteleschilonis(Munakata)生物学特性的影响。连续取食转sck+cry1Ac基因水稻的二化螟,体重下降、死亡率上升,从第2天开始,其体重显著低于取食明恢86的对照组;从第6天开始,死亡率极显著高于对照组。二化螟取食MSB36h后移至对照水稻上继续取食3、6、9、12天后,死亡率与对照差异都不显著;但体重均低于对照,其中第3天的体重差异达显著水平。二化螟绒茧蜂分别以取食MSB一定时间的3、4、5龄二化螟幼虫为寄主时,寄生率均低于以对照组,其中对4龄幼虫的寄生率差异显著;结茧率与对照差异均不显著;寄生在取食MSB的5龄二化螟幼虫体内的蜂、蛹期显著长于对照,而所结茧的茧长显著短于对照;但卵-幼虫历期、每茧块茧数、羽化率、雌性率、成蜂寿命和前翅长与对照无显著差异。结果表明转sck+cry1Ac基因水稻不仅对二化螟生长和存活有显著影响,而且可经寄主二化螟影响到二化螟绒茧蜂的一些生物学特性。

Effect of transgenic sck ＋ cry1 Ac rice （MSB） on mortality and fresh weight of the stripped stem borer （SSB）, Chilo suppressalis （Walker）, and biological characteristics of Apanteles chilonis （Munakata） via its host SSB was studied in the laboratory. Mortalities of SSB feeding on MSB increased steadily, and were significantly higher than those feeding on MH86 （CK） on day 6 and afterwards. The fresh weights of SSB continuously feeding on MSB were significantly less than those of feeding on CK on day 2 and afterwards. Mortalities of SSB feeding on CK for 3, 6, 9, 12 days after feeding on MSB for 36 h were not significantly different from those of continuously feeding on CK; but the fresh weights of the SSB were very significantly less than those feeding on CK on day 3. Parasitism rate of 4th instar larva feeding on MSB by A. chilonis was significantly lower than that of the control. Pupal duration and length of cocoon of the parasitoid from the 5th instar larva feeding on MSB were significantly longer and shorter than those of the control, respectively. However, no differences were detected in percentage of pupa formation, duration of egg and larva, cocoon number of simple cocoon mass, wasp emergence rate, female rate, longevity and forewing length of wasp between the parasitoids from SSB feeding on MSB and MH86. The results indicated that the transgenic sck＋cry1Ac rice did affect not only the mortality and body weight of the stripped stem borer, but also some biological characteristics of its parasitoid A. chilonis.