金鸡纳生物碱电喷雾质谱裂解规律研究

Study on the Dissociation Laws of Four Alkaloids in Cinchona by Electrospray Ionization MS

利用电喷雾离子阱质谱(ES-ITMS)技术进行了金鸡纳中4种喹啉类生物碱(奎宁、奎尼定、辛可宁和辛可尼定)的电喷雾质谱研究。在优化的质谱条件下,正离子全扫描时,4种生物碱都易形成带一个质子的分子离子;进一步碰撞诱导解离四种母离子,二级质谱表明羟基丢失、奎宁环断裂及重排为其主要裂解方式。

The MS behaviors and dissociation laws of four alkaloids in Cinchona were studied by direct infusion into electrospray ion trap mass spectrometry （ESI-ITMS）. The analytes （dissolved in methanol） easily formed molecular ions （[M＋H]^＋） in positive detection mode. In CID detection mode, the simple substituents such as -OH were easy to loss in the four alkaloids, and the dissociation and rearrangement of quinuclidine were also the primary behaviors of those analytes.