高糖摄入对大鼠血压、血糖、血清胰岛素及一氧化氮和血浆内皮素-1的影响

EFFECTS OF HIGH SUCROSE FEEDING ON BLOOD PRESSURE, GLUCOSE, SERUM INSULIN, NITRIC OXIDE, AND PLASMA ENDOTHELIN-1 IN RATS

①目的观察高糖摄人对大鼠血压、血糖（GLU）、血清胰岛素（INS）及一氧化氮（NO）和血浆内皮素-1（ET-1）的影响并探讨其可能的机制。②方法将雄性SD大鼠随机分为对照组（A组）和蔗糖组（B组），每组12只。A组给予正常饮用水，B组饮用水中加入120g／L蔗糖。两组均饲标准大鼠饲料。每周定时测量各组大鼠体质量、收缩压及心率1次，连续6周，实验最后一天取血，测定GLU、INS、NO及血浆ET-1等水平。③结果实验2～6周，B组血压和心率明显高于A组（t=2．91～7．74，P〈0．01）；B组与A组比较，血清GLU、INS及血浆ET-1等指标均明显升高（t=2．95～7．01，P〈0．01），而血清NO降低（t=3．01，P〈0．01）。④结论高糖摄人可诱发SD大鼠血压升高，其机制可能与胰岛素和血浆ET-1的升高及NO水平降低等有关。

Objective To investigate the effects of high sucrose feeding on blood pressure, glucose, serum insulin and nitric oxide, and plasma endothelin-1 in Sprague-Dawley （SD） rats. Methods Twenty-four male SD rats were randomly divided into two groups： control group（Group A, n=12） and sucrose group（Group B, n=12）. The rats in Group B were given 120g/L sucrose dissolved in drinking water for six weeks. Systolic blood pressure, heart rate and body weight were measured weekly. Blood samples were taken on the last day. The serum glucose, insulin, nitric oxide and plasma endothein 1 were measured. Results The rats in Group B exhibited hypertension and tachycardia compared with Group A from the second week to the end of the experiment（t=2.91-7.74,P〈0.01）. Compared with the rats in Group A, those in Group B exhibited elevated hyperglycermia, hyperinsulin, and plasma ET-1（t=2.95-7.01,P〈0.01） but lower serum NO（t=3.01, P〈0.01）. Conclusion Hypertension can be induced by high sucrose feeding in SD rats, whose mechanism is related with elevated hyperinsulin and ET-1 and lower NO.