荸荠的组织培养和快速繁殖

Tissue culture and rapid propagation of water-chestnut(Eleocharis tuberosa)

选取优质高产的荸荠品种芳林种芽为外植体进行离体培养.结果显示:以MS为基本培养基,加6-BA1.0～1.5mg/L、琼脂4.0g/L、白糖30g/L,组培苗幼芽增殖系数为3～4;而采用相同浓度培养基成分,不加琼脂的液体培养方法,组培苗幼芽增殖系数为6～8,而且植株生长整齐健壮,叶色青绿,叶状茎也长得又快又高.

The shoot tips of Chinese water -chestnut （ Eleocharis tuberosa Roem. et Schult） cultivars Fanglin were used as explants in the test of tissue culture and rapid propagation. The results showed that the proliferated coefficient of the plumules was 3-4 on MS medium containing 1.0-1.5mg/L 6 -BA, 4.0g/L agar and 30 g/L sugar.But the coefficient reached 6-8 on liquid medium with the same components except agar. The culture with the liquid medium was not only getting higher proliferated coefficient than the solid one, but also getting the seedling that grew orderly and vigorously with fresh green leaves and higher leaf-like stems.