鸡肠黏膜乳酸菌的抗菌能力及其对抗菌素的药敏特性

Inhibitory Effect of Lactic Acid Bacteria from Intestinal Mucous of Broilers and Their Susceptibility to Antibiotics

从肉鸡空肠黏膜上分离到6株菌,均为革兰氏阳性菌,其中3株乳杆菌(R2、R4、R6),3株肠球菌(R1、R3、R5)。生理生化鉴定表明,R2为嗜酸乳杆菌;R4为德氏乳杆菌乳亚种;R6为德氏乳杆菌德氏亚种;R1、R3和R5为粪肠球菌。产乳酸能力分析表明,培养至12h时R2产乳酸速度最快,到24h时R4产生乳酸浓度最高,为69.59mmol/L。在抑菌试验中,R2、R4对鸡大肠杆菌O1、鸡伤寒沙门氏菌O9和金黄色葡萄球菌的抑制作用显著高于其它菌株(P<0.05);药敏试验结果表明,R2和R4对治疗性抗菌素青霉素、四环素、卡那霉素、链霉素、万古霉素有较强的耐受性;对添加剂用抗菌素二硝托胺、硫酸多粘菌素、氨苯胂酸、杆菌肽锌的耐受性较强。

A group of six lactic acid bacteria （R1, R2, R3, R4 RS, R6） were isolated from intestinal mucous of broilers. According to their morphology, physiological and biochemical characteristics,three strains were identified as belonging to the genus of Lactobacillus： R2 as Lactobacillus acidophilus, R4 as Lactobacillus delbrueckii subsp, lactic, R6 as Lactobacillus delbrueckii subsp, delbrueckii.Other three strains were identified as Enterococcus faecalis. Lactic acid production of the six strains were investigated. The results showed that among all strains, R2 showed the most rapid production of lactic acid before 12 h. R4 showed the highest production of lactic acid in the culture by 24 h, with the concentration of lactic acid as 69. 59 mmol/L. The inhibitory effect of R2 and R4 was more significant than the other strains. R2 and R4 showed resistance to penicillin, tetracycline, streptomycin, kanamycin, vancomycin, Zoalene, Polymyxin E Sulfate, arsanilic acid and Bacitracin Zinc.