静磁场对牙周炎大鼠牙周膜组织中骨形成蛋白-2影响的实验研究

Effect of Static Magnetic Field on Bone Morphogenetic Protein-2 in Periodontal Membrane of Experimental Periodontitis Rat

目的观察静磁场对实验性牙周炎大鼠牙周膜组织中骨形成蛋白-2(BMP-2)表达的影响。方法36只大鼠牙周结扎、高糖喂食5周,确定形成牙周炎模型后,分为2组,每组18只。实验组在大鼠双侧颊部皮下组织区埋入表面磁场强度为0.12T的磁体,实验对照组埋入不充磁的磁体。2组大鼠分别于术后的第2、4、7天处死,切取牙周炎患牙及其周围牙周组织,采用SABC免疫组化方法检测病变区牙周膜组织中BMP-2的变化。同时设正常对照组。结果静磁场作用后,牙周膜组织中BMP-2表达增多,第4天和第7天时与实验对照组间存在显著性差异(P<0.05)。结论静磁场能够促进牙周炎牙周膜细胞分泌BMP-2,从而参与牙周组织的修复与重建。

Objective To evaluate the effect of statie magnetic field on expression of bone morphogenefie protein-2（BMP-2） in the periodontal membrane of experimental periodonilils rat. Methods Experimental periodonilils of rat was formed by ligaturing the neck of rat teeth and feeding sugar of high concentration. The magnet which the intensity of magnetic field was 0.12 tesla was put into their cheeks. The rats were sacrificed at 2^nd day, 4^th day and 7^th day, respectively. Immuno-histochemical techniques were used to evaluate the change of BMP-2 expression. Results BMP-2 was mainly found in the plasma of fibroblast, osteoblast,cementoeyte and odontoblast separately. There was not dearly difference between the periodonilfis group and the normal group, but BMP-2 in the experimental group treated with static magnetic field was higher than the two groups. Conclusion Static magnetic field plays an important role in repairing and remodeling of periodonilils.