锯缘青蟹精荚移植人工授精初探

Study on the artificial fertility spermatophore transplantation of the mud crab Scylla serrata

模仿雄性锯缘青蟹第一腹肢,根据其在交配中的作用机制,采用常规滴管为材料,利用酒精灯灼热拉制一个前端弯曲、侧扁、管径大小接近第一腹肢的人工交接器.以无钙离子的人工海水作为青蟹人工授精的缓冲液,缓冲液中加入适量庆大霉素.首次对青蟹采用类似硬壳交配的方式,将成熟雄蟹贮精囊内的精荚和精液成功移植至雌蟹纳精囊中.结果表明精荚的人工移植不会破坏成熟雌蟹纳精囊的内环境,其内精子具正常受精能力.未交配雌青蟹精荚的人工移植没有正常产卵,但性腺得以促进发育,并能进行正常的生殖脱皮.

Imitate male first gonopod of male mud crab, based on its function in reproduction and mate, an artificial mate organ is manufactured. The spermatophores are extended by the Ca^2＋-FSW,gentamicin is also appended in the extender. With the similar hard carapace mate methods, the spermatophores and semen are transferred to spermathacae of female mud crab artificially. As a resuit, the mature female mud crab can spawn, though the number of eggs laid is affected. The transferring in unmated female mav not spawning normally, but ovary development is accelerated and also molt.