高效液相色谱法测定舒金克喘胶囊中和厚朴酚与厚朴酚的含量

Determination of Magnolo and Honokiol in Shu Jin Ke Chuan Capsule by HPLC

目的测定舒金克喘胶囊中和厚朴酚与厚朴酚的含量。方法色谱条件色谱条件与系统适用性实验HypersilODS2C18分析柱(150mm×4.6mm,5μm);流动相甲醇∶水=(78∶22),检测波长294nm,流速1.0ml/min,室温操作。理论塔板数按厚朴酚计算应不低于3000。结果和厚朴酚与厚朴酚平均回收率分别为99.62%(RSD=1.34%),98.56%(RSD=1.49%)。结论方法快速准确,样品处理简便易行。

Objective： To determine the magnolo and honokiol in Shu Jin Ke Chuan Capsule. Methods： HPLC system consisted of Hypersil ODS 2 C18 column （125 mm×4.6mm, 5μm） Methanol：water=78 ： 22. As mobile phase, with detection wavelength at 294 nm, flow rate 1.0 ml/min. Results： For honokiol the mean recovery of 99. 62% （RSD= 1.34%）, for Magnolo 98.56% （RSD= 1.49%） for strychnine is obtained respectively. Conclusion： The method is sensitive, rapid and accurate.