旱莲草中总黄酮和鞣质的超声提取与含量测定

Ultrasonic Extraction and Determination of Total Flavonoids and Tannin in Eclipta prostrata (L.)

目的从旱莲草中提取总黄酮和鞣质,并测定其含量。方法运用超声技术提取旱莲草总黄酮和鞣质,用比色法测定总黄酮含量,配位滴定法测定鞣质的含量。结果测得旱莲草中总黄酮含量为0.704%,平均回收率为100.3%,相对标准偏差(RSD)为1.77%(n=5);鞣质含量为4.82%,相对标准偏差(RSD)为0.939%(n=3)。结论运用超声技术从旱莲草中提取总黄酮和鞣质,反应速度加快,提高了提取效率。

Objective： To extract and determine the total flavonoids and tannin in Eclipta prostrata （L）. Methods： Total flavonoids and tannin in Eclipta prostrata （L.） were extracted by ultrasonic technology, total flavonoids determined by colorimetry and tannin was determined by the method of complex titration. Results： Total flavonoids in Eclipta prostrata （L.）was 0.704%,and recovery rate was 100. 3%,RSD was 1. 77%（n=5）;Tannin in Eclipta prostrata （L.） was 4.82 %,RSD was 0. 939 % （n = 3）. Conelusion： Ultrasonic technology was applied to extracting total flavonoids and tannin in Eclipta prostrata （L.） for the first time. The reaction speed is faster and the result is satisfactory.