水稻叶尖枯病人工接种技术研究

A TECHNIQUE FOR INOCULATION OF RICE PHYLLOSTICTALEAF BLIGHT

接种试验表明，病菌菌丝液和分生孢子液均可作为接种体。凡能造成伤口的接种方法，都能使稻叶发病，其中以剪叶蘸液接种最为简便、实用。而非伤口接种方法则不行，表明病菌侵入途径主要是伤口。接种后不管保湿与否，均能发病，但保湿时间愈长，病害潜育期愈短，病斑扩展速度愈快。试验还指出，水稻苗期和分蘖期叶片较抗病，而孕穗期、扬花期和乳熟期较感病。因此，孕穗至扬花期是大田接种鉴定水稻品种抗性的适期。

The mycelia and conidia of Phyllosiicta oryzicola Hara collld be used as inocula。The rice leavescould be infected by means of wound inoculations, and in which the clipping n1ethtxl is simplest and mostpractical. But the unwounded inoculations were not successful,which was meant that P。 ory:icola penetratedrice plants only through wounds。AlI the leaves could be diseased whether they were in the condition of moisture preservatiOn or not after the inoculation, but the Ionger time for lnoisture。preservation,the shorter incubation peried of the disease, the faster spread of the lesion。otherwise,the experiment showed that the riceIeaves in the stages of seedling and tillering were resistant to the disease, but those in the stages of hoting,fIowering and n1ilk were susceptlble。 Therefore, the booting-flowering stage is a suitable perlto for the eva1ution of the resistance of rice varieties to the disease by fleld inoculation。