流动注射化学发光法测定环丙沙星

Flow Injection Analysis of Ciprofloxacin by Terbium Sensitized Chemiluminescence Method

环丙沙星(CPLX)在酸性介质中与Tb3+形成配合物, 通过能量转移反应对Ce(Ⅳ)-SO2-3化学发光体系有很强的增敏作用. 据此, 采用流动注射技术, 建立了流动注射稀土敏化化学发光法测定CPLX的新方法. 在9.0×10-9～1.0×10-6 mol·L-1范围内CPLX浓度与化学发光强度呈良好的线性关系, 方法的检出限为2.2×10-11 mol·L-1, 对5.0×10-8 mol·L-1 CPLX平行测定11次, 相对标准偏差为3.0%. 利用该法测定了胶囊和生物体液中CPLX的含量, 方法简单快速, 重现性好, 结果令人满意.

A new flow injection chemiluminescence （CL） method for the determination of Ciprofloxacin （CPLX） was described. The method was based on the weak CL reaction of Ce（Ⅳ）-SO3^2- sensitized by Tb^3＋-CPLX. The relative CL intensity was linear with the concentration of CPLX in the range of 9.0×10^-9-1.0×10^-6mol·L^-1. The detection limit is 2.2×10^-11mol·L^-1. The relative standard deviation （RSD） for the determination of 5.0×10^-8mol·L^-1 CPLX is 3.0% （n=11）. The method has been successfully applied to the analysis of CPLX in tablet and capsule. An original mechanism was proposed to explain this phenomenon.