摘要
以蒜头果(Malaniaoleifera)种子萌发获得的无菌苗进行离体培养的初步研究.结果表明:MS+6-BA1.5mg/L+NAA0.1mg/L激素组合能够较好地诱导芽的初始分化和增殖;适宜的继代增殖培养基为MS+6-BA1.0mg/L+NAA0.2mg/L;采用1/2MS+IBA0.5mg/L为生根培养基,生根率为45.0%;移栽到河沙的生根苗成活率为52.5%.
A tissue culture method is described for clonal multiplication of Malania oleifera using node stem.Results show that the blank MS medium can induct aseptic seedlings and the induction rate is 85.3 %. The suitable culture medium for inducing bud is MS supplemented with 1.5mg/L 6-BA and 0. lmg/L NAA, and for the generation-continuing multiplication is MS supplemented with 1.5mg/L 6-BA and 0.2mg/L NAA.The rooting medium is 1/2MS supplemented with 0.5mg/L IBA and the rooting rate is 45.0%. Plantlet survival after transfer to sand is 52.5%.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第4期839-843,共5页
Journal of Sichuan University(Natural Science Edition)
基金
广西大学谭锦球基金(X0102014)
关键词
蒜头果
种子
组织培养
再生植株
Malania oleifera
seed
tissue culture
regenerated plantlets
